1w3x: Difference between revisions
New page: left|200px<br /> <applet load="1w3x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w3x, resolution 1.46Å" /> '''ISOPENICILLIN N SYN... |
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==About this Structure== | ==About this Structure== | ||
1W3X is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2 and W2X as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W3X OCA]]. | 1W3X is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with FE2 and W2X as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isopenicillin-N_synthase Isopenicillin-N synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W3X OCA]]. | ||
==Reference== | ==Reference== | ||
Unexpected oxidation of a depsipeptide substrate analogue in crystalline isopenicillin N synthase., Daruzzaman A, Clifton IJ, Adlington RM, Baldwin JE, Rutledge PJ, Chembiochem. 2006 Feb;7(2):351-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16444759 16444759] | Unexpected oxidation of a depsipeptide substrate analogue in crystalline isopenicillin N synthase., Daruzzaman A, Clifton IJ, Adlington RM, Baldwin JE, Rutledge PJ, Chembiochem. 2006 Feb;7(2):351-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16444759 16444759] | ||
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Isopenicillin-N synthase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Clifton, I.J.]] | [[Category: Clifton, I.J.]] | ||
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[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:42:29 2007'' | ||
Revision as of 12:37, 30 October 2007
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ISOPENICILLIN N SYNTHASE D-(L-A-AMINOADIPOYL)-(3R)-METHYL-L-CYSTEINE D-A-HYDROXYISOVALERYL ESTER COMPLEX (OXYGEN EXPOSED 5 MINUTES 20 BAR)
OverviewOverview
Isopenicillin N synthase (IPNS) is a non-heme iron(ii)-dependent oxidase, that is central to penicillin biosynthesis. Herein, we report mechanistic, studies of the IPNS reaction in the crystalline state, using the substrate, analogue delta-(L-alpha-aminoadipoyl)-(3R)-methyl-L-cysteine, D-alpha-hydroxyisovaleryl ester (AmCOV) to probe the early stages of the, catalytic cycle. The X-ray crystal structure of the anaerobic, IPNS:Fe(II):AmCOV complex was solved to 1.40 A resolution, and it reveals, several subtle differences in the active site relative to the complex of, the enzyme with its natural substrate. The crystalline IPNS:Fe(II):AmCOV, complex was then exposed to oxygen gas at high pressure; this brought, about reaction to give what appears to be a hydroxymethyl/ene-thiol, product. A ... [(full description)]
About this StructureAbout this Structure
1W3X is a [Single protein] structure of sequence from [Emericella nidulans] with FE2 and W2X as [ligands]. Active as [Isopenicillin-N synthase], with EC number [1.21.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Unexpected oxidation of a depsipeptide substrate analogue in crystalline isopenicillin N synthase., Daruzzaman A, Clifton IJ, Adlington RM, Baldwin JE, Rutledge PJ, Chembiochem. 2006 Feb;7(2):351-8. PMID:16444759
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