1uwz: Difference between revisions
New page: left|200px<br /> <applet load="1uwz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uwz, resolution 1.99Å" /> '''BACILLUS SUBTILIS C... |
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==About this Structure== | ==About this Structure== | ||
1UWZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with ZN and THU as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.5 3.5.4.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UWZ OCA]]. | 1UWZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with ZN and THU as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cytidine_deaminase Cytidine deaminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.5 3.5.4.5]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UWZ OCA]]. | ||
==Reference== | ==Reference== | ||
Structural, kinetic, and mutational studies of the zinc ion environment in tetrameric cytidine deaminase., Johansson E, Neuhard J, Willemoes M, Larsen S, Biochemistry. 2004 May 25;43(20):6020-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15147186 15147186] | Structural, kinetic, and mutational studies of the zinc ion environment in tetrameric cytidine deaminase., Johansson E, Neuhard J, Willemoes M, Larsen S, Biochemistry. 2004 May 25;43(20):6020-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15147186 15147186] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Cytidine deaminase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Johansson, E.]] | [[Category: Johansson, E.]] | ||
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[[Category: zinc binding]] | [[Category: zinc binding]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:00:21 2007'' | ||
Revision as of 11:55, 30 October 2007
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BACILLUS SUBTILIS CYTIDINE DEAMINASE WITH AN ARG56- ALA SUBSTITUTION
OverviewOverview
The zinc-containing cytidine deaminase (CDA, EC 3.5.4.5) is a pyrimidine, salvage enzyme catalyzing the hydrolytic deamination of cytidine and, 2'-deoxycytidine forming uridine and 2'-deoxyuridine, respectively., Homodimeric CDA (D-CDA) and homotetrameric CDA (T-CDA) both contain one, zinc ion per subunit coordinated to the catalytic water molecule. The zinc, ligands in D-CDA are one histidine and two cysteine residues, whereas in, T-CDA zinc is coordinated to three cysteines. Two of the zinc coordinating, cysteines in T-CDA form hydrogen bonds to the conserved residue Arg56, and, this residue together with the dipole moments from two alpha-helices, partially neutralizes the additional negative charge in the active site, leading to a catalytic activity similar to D-CDA. Arg56 has been, ... [(full description)]
About this StructureAbout this Structure
1UWZ is a [Single protein] structure of sequence from [Bacillus subtilis] with ZN and THU as [ligands]. Active as [Cytidine deaminase], with EC number [3.5.4.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structural, kinetic, and mutational studies of the zinc ion environment in tetrameric cytidine deaminase., Johansson E, Neuhard J, Willemoes M, Larsen S, Biochemistry. 2004 May 25;43(20):6020-9. PMID:15147186
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