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==CRYSTAL STRUCTURE OF GENETICALLY DETOXIFIED PERTUSSIS TOXIN GDPT.==
==CRYSTAL STRUCTURE OF GENETICALLY DETOXIFIED PERTUSSIS TOXIN GDPT.==
<StructureSection load='6ro0' size='340' side='right'caption='[[6ro0]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
<StructureSection load='6ro0' size='340' side='right'caption='[[6ro0]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6ro0]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RO0 FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RO0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13&#8491;</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ro0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ro0 OCA], [https://pdbe.org/6ro0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ro0 RCSB], [https://www.ebi.ac.uk/pdbsum/6ro0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ro0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ro0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ro0 OCA], [https://pdbe.org/6ro0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ro0 RCSB], [https://www.ebi.ac.uk/pdbsum/6ro0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ro0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TOX3_BORPE TOX3_BORPE] PTX oligomer B binds to receptors on the eukaryotic cell surface and facilitates the translocation of the toxic subunit across the cell membrane.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mutant gdPT R9K/E129G is a genetically detoxified variant of the pertussis toxin (PTx) and represents an attractive candidate for the development of improved pertussis vaccines. The impact of the mutations on the overall protein structure and its immunogenicity has remained elusive. Here we present the crystal structure of gdPT and show that it is nearly identical to that of PTx. Hydrogen-deuterium exchange mass spectrometry revealed dynamic changes in the catalytic domain that directly impacted NAD(+) binding which was confirmed by biolayer interferometry. Distal changes in dynamics were also detected in S2-S5 subunit interactions resulting in tighter packing of B-oligomer corresponding to increased thermal stability. Finally, antigen stimulation of human whole blood, analyzed by a previously unreported mass cytometry assay, indicated broader immunogenicity of gdPT compared to pertussis toxoid. These findings establish a direct link between the conserved structure of gdPT and its ability to generate a robust immune response.
Genetically detoxified pertussis toxin displays near identical structure to its wild-type and exhibits robust immunogenicity.,Ausar SF, Zhu S, Duprez J, Cohen M, Bertrand T, Steier V, Wilson DJ, Li S, Sheung A, Brookes RH, Pedyczak A, Rak A, Andrew James D Commun Biol. 2020 Aug 5;3(1):427. doi: 10.1038/s42003-020-01153-3. PMID:32759959<ref>PMID:32759959</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ro0" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Pertussis toxin|Pertussis toxin]]
*[[Pertussis toxin|Pertussis toxin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bordetella pertussis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bertrand T]]
[[Category: Bertrand, T]]

Latest revision as of 11:17, 17 October 2024

CRYSTAL STRUCTURE OF GENETICALLY DETOXIFIED PERTUSSIS TOXIN GDPT.CRYSTAL STRUCTURE OF GENETICALLY DETOXIFIED PERTUSSIS TOXIN GDPT.

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

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