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==CryoEM structure of RuvA-RuvB-Holliday junction complex== | |||
<StructureSection load='7x7q' size='340' side='right'caption='[[7x7q]], [[Resolution|resolution]] 7.02Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7x7q]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X7Q FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x7q OCA], [https://pdbe.org/7x7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x7q RCSB], [https://www.ebi.ac.uk/pdbsum/7x7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x7q ProSAT]</span></td></tr> | |||
[[Category: | </table> | ||
[[Category: | == Function == | ||
[[Category: Lin | [https://www.uniprot.org/uniprot/RUVA_PSEAE RUVA_PSEAE] The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA (By similarity). Complements an E.coli deletion mutant (PubMed:8982068).[HAMAP-Rule:MF_00031]<ref>PMID:8982068</ref> | ||
[[Category: Zhang | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa PAO1]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Lin Z]] | |||
[[Category: Qu Q]] | |||
[[Category: Zhang X]] | |||
[[Category: Zhou Z]] | |||
Revision as of 12:49, 15 March 2023
CryoEM structure of RuvA-RuvB-Holliday junction complexCryoEM structure of RuvA-RuvB-Holliday junction complex
Structural highlights
FunctionRUVA_PSEAE The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA (By similarity). Complements an E.coli deletion mutant (PubMed:8982068).[HAMAP-Rule:MF_00031][1] References
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