7x7q

CryoEM structure of RuvA-RuvB-Holliday junction complexCryoEM structure of RuvA-RuvB-Holliday junction complex

Structural highlights

7x7q is a 16 chain structure with sequence from Pseudomonas aeruginosa PAO1 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 7.02Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RUVA_PSEAE The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA (By similarity). Complements an E.coli deletion mutant (PubMed:8982068).[HAMAP-Rule:MF_00031]^[1]

References

↑ Hishida T, Iwasaki H, Ishioka K, Shinagawa H. Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates. Gene. 1996 Dec 5;182(1-2):63-70. PMID:8982068 doi:10.1016/s0378-1119(96)00474-x

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OCA

[1] Hishida T, Iwasaki H, Ishioka K, Shinagawa H. Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates. Gene. 1996 Dec 5;182(1-2):63-70. PMID:8982068 doi:10.1016/s0378-1119(96)00474-x

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