5nd4: Difference between revisions

Latest revision as of 15:48, 15 November 2023

Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFxMicrotubule-bound MKLP2 motor domain in the presence of ADP.AlFx

5nd4, resolution 4.40Å

Structural highlights

5nd4 is a 3 chain structure with sequence from Bos taurus and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 4.4Å
Ligands:
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KI20A_MOUSE Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle (By similarity). Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.^[1]

Publication Abstract from PubMed

MKLP2, a kinesin-6, has critical roles during the metaphase-anaphase transition and cytokinesis. Its motor domain contains conserved nucleotide binding motifs, but is divergent in sequence (~35% identity) and size (~40% larger) compared to other kinesins. Using cryo-electron microscopy and biophysical assays, we have undertaken a mechanochemical dissection of the microtubule-bound MKLP2 motor domain during its ATPase cycle, and show that many facets of its mechanism are distinct from other kinesins. While the MKLP2 neck-linker is directed towards the microtubule plus-end in an ATP-like state, it does not fully dock along the motor domain. Furthermore, the footprint of the MKLP2 motor domain on the MT surface is altered compared to motile kinesins, and enhanced by kinesin-6-specific sequences. The conformation of the highly extended loop6 insertion characteristic of kinesin-6s is nucleotide-independent and does not contact the MT surface. Our results emphasize the role of family-specific insertions in modulating kinesin motor function.

The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry.,Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA Elife. 2017 Aug 11;6. pii: e27793. doi: 10.7554/eLife.27793. PMID:28826477^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Echard A, Jollivet F, Martinez O, Lacapere JJ, Rousselet A, Janoueix-Lerosey I, Goud B. Interaction of a Golgi-associated kinesin-like protein with Rab6. Science. 1998 Jan 23;279(5350):580-5. PMID:9438855
↑ Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA. The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry. Elife. 2017 Aug 11;6. pii: e27793. doi: 10.7554/eLife.27793. PMID:28826477 doi:http://dx.doi.org/10.7554/eLife.27793

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OCA

[1] Echard A, Jollivet F, Martinez O, Lacapere JJ, Rousselet A, Janoueix-Lerosey I, Goud B. Interaction of a Golgi-associated kinesin-like protein with Rab6. Science. 1998 Jan 23;279(5350):580-5. PMID:9438855

[2] Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA. The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry. Elife. 2017 Aug 11;6. pii: e27793. doi: 10.7554/eLife.27793. PMID:28826477 doi:http://dx.doi.org/10.7554/eLife.27793

[1]

[2]

@@ Line 3: / Line 3: @@
 <SX load='5nd4' size='340' side='right' viewer='molstar' caption='[[5nd4]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5nd4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ND4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ND4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5nd4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ND4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ND4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nd4 OCA], [http://pdbe.org/5nd4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nd4 RCSB], [http://www.ebi.ac.uk/pdbsum/5nd4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nd4 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nd4 OCA], [https://pdbe.org/5nd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nd4 RCSB], [https://www.ebi.ac.uk/pdbsum/5nd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nd4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KI20A_MOUSE KI20A_MOUSE]] Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle (By similarity). Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.<ref>PMID:9438855</ref>  [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/F2Z4C1_BOVIN F2Z4C1_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00824325]
+[https://www.uniprot.org/uniprot/KI20A_MOUSE KI20A_MOUSE] Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle (By similarity). Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.<ref>PMID:9438855</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 ==See Also==
 *[[Kinesin 3D Structures|Kinesin 3D Structures]]
-*[[Tubulin 3D Structures|Tubulin 3D Structures]]
 == References ==
 <references/>
@@ Line 28: / Line 28: @@
 [[Category: Bos taurus]]
 [[Category: Large Structures]]
-[[Category: Atherton, J]]
+[[Category: Mus musculus]]
-[[Category: Clause, J]]
+[[Category: Atherton J]]
-[[Category: Cook, A]]
+[[Category: Clause J]]
-[[Category: Houdusse, A]]
+[[Category: Cook A]]
-[[Category: Joseph, A P]]
+[[Category: Houdusse A]]
-[[Category: Major, J]]
+[[Category: Joseph AP]]
-[[Category: Moores, C A]]
+[[Category: Major J]]
-[[Category: Muretta, J M]]
+[[Category: Moores CA]]
-[[Category: Rosenfeld, S S]]
+[[Category: Muretta JM]]
-[[Category: Sourigues, Y]]
+[[Category: Rosenfeld SS]]
-[[Category: Topf, M]]
+[[Category: Sourigues Y]]
-[[Category: Yu, I M]]
+[[Category: Topf M]]
-[[Category: Kinesin]]
+[[Category: Yu I-M]]
-[[Category: Microtubule]]
-[[Category: Mitosis]]
-[[Category: Motor protein]]

5nd4: Difference between revisions

Latest revision as of 15:48, 15 November 2023

Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFxMicrotubule-bound MKLP2 motor domain in the presence of ADP.AlFx

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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5nd4: Difference between revisions

Latest revision as of 15:48, 15 November 2023

Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFxMicrotubule-bound MKLP2 motor domain in the presence of ADP.AlFx

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

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