4pko: Difference between revisions
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<StructureSection load='4pko' size='340' side='right'caption='[[4pko]], [[Resolution|resolution]] 3.84Å' scene=''> | <StructureSection load='4pko' size='340' side='right'caption='[[4pko]], [[Resolution|resolution]] 3.84Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4pko]] is a 28 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4pko]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKO FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pko OCA], [https://pdbe.org/4pko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pko RCSB], [https://www.ebi.ac.uk/pdbsum/4pko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pko ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q548M1_ECOLX Q548M1_ECOLX] Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity).[RuleBase:RU000419][HAMAP-Rule:MF_00600] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Chaperonin|Chaperonin]] | *[[Chaperonin 3D structures|Chaperonin 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fei | [[Category: Fei X]] | ||
[[Category: Laronde-Leblanc | [[Category: Laronde-Leblanc N]] | ||
[[Category: Lorimer | [[Category: Lorimer GH]] | ||
[[Category: Ye | [[Category: Ye X]] | ||
Revision as of 10:16, 8 February 2023
Crystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complexCrystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complex
Structural highlights
FunctionQ548M1_ECOLX Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity).[RuleBase:RU000419][HAMAP-Rule:MF_00600] Publication Abstract from PubMedThe GroE chaperonins assist substrate protein (SP) folding by cycling through several conformational states. With each cycle the SP is, in turn, captured, unfolded, briefly encapsulated (t1/2 approximately 1 s), and released by the chaperonin complex. The protein-folding functional form is the US-football-shaped GroEL:GroES2 complex. We report structures of two such "football" complexes to approximately 3.7-A resolution; one is empty whereas the other contains encapsulated SP in both chambers. Although encapsulated SP is not visible on the electron density map, using calibrated FRET and order-of-addition experiments we show that owing to SP-catalyzed ADP/ATP exchange both chambers of the football complex encapsulate SP efficiently only if the binding of SP precedes that of ATP. The two rings of GroEL thus behave as a parallel processing machine, rather than functioning alternately. Compared with the bullet-shaped GroEL:GroES1 complex, the GroEL:GroES2 football complex differs conformationally at the GroEL-GroES interface and also at the interface between the two GroEL rings. We propose that the electrostatic interactions between the epsilon-NH3+ of K105 of helix D in one ring with the negatively charged carboxyl oxygen of A109 at the carboxyl end of helix D of the other ring provide the structural basis for negative inter-ring cooperativity. Formation and structures of GroEL:GroES2 chaperonin footballs, the protein-folding functional form.,Fei X, Ye X, LaRonde NA, Lorimer GH Proc Natl Acad Sci U S A. 2014 Aug 18. pii: 201412922. PMID:25136110[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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