4pko

Crystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complexCrystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complex

Structural highlights 4pko is a 28 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.84Å Ligands: , , , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q7BGE6_ECOLX Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter (By similarity).[SAAS:SAAS018369_004_006304][RuleBase:RU000535][HAMAP-Rule:MF_00580] Publication Abstract from PubMed The GroE chaperonins assist substrate protein (SP) folding by cycling through several conformational states. With each cycle the SP is, in turn, captured, unfolded, briefly encapsulated (t1/2 approximately 1 s), and released by the chaperonin complex. The protein-folding functional form is the US-football-shaped GroEL:GroES2 complex. We report structures of two such "football" complexes to approximately 3.7-A resolution; one is empty whereas the other contains encapsulated SP in both chambers. Although encapsulated SP is not visible on the electron density map, using calibrated FRET and order-of-addition experiments we show that owing to SP-catalyzed ADP/ATP exchange both chambers of the football complex encapsulate SP efficiently only if the binding of SP precedes that of ATP. The two rings of GroEL thus behave as a parallel processing machine, rather than functioning alternately. Compared with the bullet-shaped GroEL:GroES1 complex, the GroEL:GroES2 football complex differs conformationally at the GroEL-GroES interface and also at the interface between the two GroEL rings. We propose that the electrostatic interactions between the epsilon-NH3+ of K105 of helix D in one ring with the negatively charged carboxyl oxygen of A109 at the carboxyl end of helix D of the other ring provide the structural basis for negative inter-ring cooperativity. Formation and structures of GroEL:GroES2 chaperonin footballs, the protein-folding functional form.,Fei X, Ye X, LaRonde NA, Lorimer GH Proc Natl Acad Sci U S A. 2014 Aug 18. pii: 201412922. PMID:25136110 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Chaperonin 3D structures

spinqualitylabelsall models 4pko, resolution 3.84Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image