1is7: Difference between revisions
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==Crystal structure of rat GTPCHI/GFRP stimulatory complex== | ==Crystal structure of rat GTPCHI/GFRP stimulatory complex== | ||
<StructureSection load='1is7' size='340' side='right' caption='[[1is7]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='1is7' size='340' side='right'caption='[[1is7]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1is7]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IS7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1is7]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IS7 FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1is7" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1is7" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Cyclohydrolase 3D structures|Cyclohydrolase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Buffalo rat]] | [[Category: Buffalo rat]] | ||
[[Category: GTP cyclohydrolase I]] | [[Category: GTP cyclohydrolase I]] | ||
[[Category: Large Structures]] | |||
[[Category: Hakoshima, T]] | [[Category: Hakoshima, T]] | ||
[[Category: Hatakeyama, K]] | [[Category: Hatakeyama, K]] | ||
Revision as of 12:57, 30 October 2019
Crystal structure of rat GTPCHI/GFRP stimulatory complexCrystal structure of rat GTPCHI/GFRP stimulatory complex
Structural highlights
Function[GCH1_RAT] May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis (By similarity). May modify pain sensitivity and persistence.[1] [GFRP_RAT] Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedIn the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers. Each GFRP pentamer forms a symmetrical five-membered ring similar to beta-propeller. Five phenylalanine molecules are buried inside each interface between GFRP and GTPCHI, thus enhancing the binding of these proteins. The complex structure suggests that phenylalanine-induced GTPCHI x GFRP complex formation enhances GTPCHI activity by locking the enzyme in the active state. Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP.,Maita N, Okada K, Hatakeyama K, Hakoshima T Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1212-7. Epub 2002 Jan 29. PMID:11818540[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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