UvrABC: Difference between revisions

Revision as of 01:06, 14 October 2017

Function

UvrABC endonuclease is an E. coli enzyme complex involved in DNA repair. UvrABC removes 12 nucleotides around a DNA mutation replacing them with the correct one^[1].

UvrA is the protein which locates the DNA damage.
UvrB is involved in distinguishing damaged from undamaged DNA^[2].
The C-terminal region of UvrC is involved in DNA binding and incisions at the 5'-side of a DNA damage during nucleotide excision repair^[3].
UvrD is DNA helicase II. See Helicase.

For details on the UvrA-UvrB complex see UvrA-UvrB interaction domains.

Structural highlights

UvrA contains several domains: ^[4].

3D structures of UvrABC3D structures of UvrABC

Updated on 14-October-2017

ReferencesReferences

↑ Moolenaar GF, Moorman C, Goosen N. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J Bacteriol. 2000 Oct;182(20):5706-14. PMID:11004168
↑ Theis K, Skorvaga M, Machius M, Nakagawa N, Van Houten B, Kisker C. The nucleotide excision repair protein UvrB, a helicase-like enzyme with a catch. Mutat Res. 2000 Aug 30;460(3-4):277-300. PMID:10946234
↑ Moolenaar GF, Uiterkamp RS, Zwijnenburg DA, Goosen N. The C-terminal region of the Escherichia coli UvrC protein, which is homologous to the C-terminal region of the human ERCC1 protein, is involved in DNA binding and 5'-incision. Nucleic Acids Res. 1998 Jan 15;26(2):462-8. PMID:9421501
↑ Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Moolenaar GF, Moorman C, Goosen N. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J Bacteriol. 2000 Oct;182(20):5706-14. PMID:11004168

[2] Theis K, Skorvaga M, Machius M, Nakagawa N, Van Houten B, Kisker C. The nucleotide excision repair protein UvrB, a helicase-like enzyme with a catch. Mutat Res. 2000 Aug 30;460(3-4):277-300. PMID:10946234

[3] Moolenaar GF, Uiterkamp RS, Zwijnenburg DA, Goosen N. The C-terminal region of the Escherichia coli UvrC protein, which is homologous to the C-terminal region of the human ERCC1 protein, is involved in DNA binding and 5'-incision. Nucleic Acids Res. 1998 Jan 15;26(2):462-8. PMID:9421501

[4] Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973

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@@ Line 24: / Line 24: @@
 **[[2r6f]] – BstUvrA – ''Bacillus stearothermophilus'' <br />
 **[[3ux8]] – GeUvrA - ''Geobacillus''<br />
+**[[2vf7]], [[2vf8]] – DrUvrA2 - ''Deinococcus radiodurans''<br />
 *UvrB
@@ Line 38: / Line 39: @@
 *UvrC
-**[[1kft]] – EcUvrC C terminal <br />
+**[[1kft]], [[1qoj]] – EcUvrC C terminal <br />
+**[[1e52]] – EcUvrC C terminal - NMR<br />
 **[[2nrr]], [[2nrt]], [[2nrv]], [[2nrw]], [[2nrx]], [[2nrz]] – TmUvrC C terminal <br />
 **[[1ycz]], [[1yd0]], [[1yd1]] – TmUvrC N terminal <br />
@@ Line 44: / Line 46: @@
 **[[1yd6]] – BcUvrC N terminal <br />
 **[[3c65]] – BstUvrC  <br />
+*UvrD or DNA helicase II
+**[[3lfu]] – EcUvrD <br />
+**[[2is1]], [[2is2]] – EcUvrD (mutant) + DNA<br />
+**[[2is4]] – EcUvrD (mutant) + DNA + ADPNP<br />
+**[[2is6]] – EcUvrD (mutant) + DNA + ADP<br />
+**[[4c2u]] – DrUvrD + DNA<br />
+**[[4c30]] – DrUvrD + DNA + ADPNP<br />
+**[[4c2t]] – DrUvrD (mutant) + DNA + ADPNP<br />
 *UvrA-UvrB