3aq2: Difference between revisions
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==Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b== | ==Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b== | ||
<StructureSection load='3aq2' size='340' side='right' caption='[[3aq2]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='3aq2' size='340' side='right'caption='[[3aq2]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3aq2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrvi Agrvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQ2 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[3aq2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrvi Agrvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQ2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3AQ2 FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aq3|3aq3]], [[3aq4|3aq4]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aq3|3aq3]], [[3aq4|3aq4]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3aq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aq2 OCA], [http://pdbe.org/3aq2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aq2 RCSB], [http://www.ebi.ac.uk/pdbsum/3aq2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aq2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Agrvi]] | [[Category: Agrvi]] | ||
[[Category: Large Structures]] | |||
[[Category: Wang, M M]] | [[Category: Wang, M M]] | ||
[[Category: Yuan, Y A]] | [[Category: Yuan, Y A]] | ||
Revision as of 15:24, 29 July 2020
Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6bMolecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b
Structural highlights
Publication Abstract from PubMedThe Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 A, Agrobacterium vitis AB6b at 1.65 A, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 A. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity. Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b.,Wang M, Soyano T, Machida S, Yang JY, Jung C, Chua NH, Yuan YA Genes Dev. 2011 Jan 1;25(1):64-76. Epub 2010 Dec 14. PMID:21156810[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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