3evv: Difference between revisions
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<StructureSection load='3evv' size='340' side='right' caption='[[3evv]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='3evv' size='340' side='right' caption='[[3evv]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3evv]] is a 1 chain structure | <table><tr><td colspan='2'>[[3evv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EVV FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3evp|3evp]], [[3evr|3evr]], [[3evu|3evu]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3evp|3evp]], [[3evr|3evr]], [[3evu|3evu]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3evv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3evv OCA], [http://pdbe.org/3evv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3evv RCSB], [http://www.ebi.ac.uk/pdbsum/3evv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3evv ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3evv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3evv OCA], [http://pdbe.org/3evv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3evv RCSB], [http://www.ebi.ac.uk/pdbsum/3evv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3evv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Kotlikoff, M I]] | |||
[[Category: Kotlikoff,M I]] | [[Category: Shui, B]] | ||
[[Category: Shui,B]] | [[Category: Sondermann, H]] | ||
[[Category: Sondermann,H]] | [[Category: Wang, Q]] | ||
[[Category: Wang,Q]] | |||
[[Category: Calcium sensor]] | [[Category: Calcium sensor]] | ||
[[Category: Calmodulin]] | [[Category: Calmodulin]] | ||
Revision as of 12:18, 3 August 2017
Crystal Structure of Calcium bound dimeric GCAMP2 (#2)Crystal Structure of Calcium bound dimeric GCAMP2 (#2)
Structural highlights
Publication Abstract from PubMedGenetically encoded Ca(2+) indicators are important tools that enable the measurement of Ca(2+) dynamics in a physiologically relevant context. GCaMP2, one of the most robust indicators, is a circularly permutated EGFP (cpEGFP)/M13/calmodulin (CaM) fusion protein that has been successfully used for studying Ca(2+) fluxes in vivo in the heart and vasculature of transgenic mice. Here we describe crystal structures of bright and dim states of GCaMP2 that reveal a sophisticated molecular mechanism for Ca(2+) sensing. In the bright state, CaM stabilizes the fluorophore in an ionized state similar to that observed in EGFP. Mutational analysis confirmed critical interactions between the fluorophore and elements of the fused peptides. Solution scattering studies indicate that the Ca(2+)-free form of GCaMP2 is a compact, predocked state, suggesting a molecular basis for the relatively rapid signaling kinetics reported for this indicator. These studies provide a structural basis for the rational design of improved Ca(2+)-sensitive probes. Structural Basis for Calcium Sensing by GCaMP2.,Wang Q, Shui B, Kotlikoff MI, Sondermann H Structure. 2008 Dec 12;16(12):1817-27. PMID:19081058[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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