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| * '''ExN-V''' cleaves linear double-stranded DNA.<br /> | | * '''ExN-V''' cleaves linear double-stranded DNA.<br /> |
| * '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | | * '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> |
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| | <scene name='46/466467/Cv/2'>Mg coordination site</scene> in ''E. coli'' exonuclease (PDB code [[1fxx]]).<ref>PMID:11101894</ref> |
| </StructureSection> | | </StructureSection> |
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Revision as of 16:06, 14 February 2016
Exonucleases (ExN) cleave nucleotides at the end of a polynucleotide chain[1]. In E. coli there are ExN I to VIII. ExN were found in human and yeast as well.
- ExN-I cleaves single-stranded DNA.
- ExN-II is associated with DNA polymerase.
- ExN-III cleaves double-stranded DNA.
- ExN-IV cleavage results in nucleoside monophosphate.
- ExN-V cleaves linear double-stranded DNA.
- ExN-VIII requires a free 5’ OH group for reacting.
in E. coli exonuclease (PDB code 1fxx).[2]
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3D Structures of exonuclease3D Structures of exonuclease
Updated on 14-February-2016
{"openlevels":0}
- ExN-I
- 1fxx, 3c95 – EcExN-I – Escherichia coli
- 2qxf - EcExN-I + TMP
- 3hl8, 3hp9 - EcExN-I + inhibitor
- 3c94 - EcExN-I + DNA-binding C terminal peptide
- 4jrp, 4jrq – EcExN-I + oligonucleotide
- 3qe9 – hExN-I (mutant) + DNA – human
- 3qea, 3qeb - hExN-I + DNA
- 4rg8 – ExN-I (mutant) – Methylocaldum szegedienes
- ExN-III
- ExN-IV
- 2jc4 – ExN-IV – Nisseria meningitides
- ExN-V
- 1w36, 3k70 – EcExN-V α+β+γ + DNA hairpin
- 3e1s - EcExN-V RECD subunit
- 3gp8 - DrExN-V RECD subunit + DNA – Deinococcus radiodurans
- 3gpl - DrExN-V RECD subunit + DNA + ADPNP
- ExN-VII
- 1vp7 – ExN-VII small subunit – Bordetella pertussis
- ExN-VIII
- 3h4r - EcExN-VIII C terminal (mutant)
- Phage ExN
- 1ut5, 1ut8 – T5-5’-ExN + metal – Bacteriophage T5
- 1exn, 1xo1 - T5-5’-ExN (mutant)
- 1avq – BlExN λ (mutant) – Bacteriophage λ
- 3slp, 4wuz - BlExN λ + DNA
- 3sm4 - BlExN λ (mutant) + DNA
- 3k93 – ExN – Haemophilus somnus
- Exosome complex ExN
- 2ba1 – AfExN Rrp41
- 2br2 – SsExN-I + ExN-II – Sulfolobus solfataricus
- 2jea, 2je6, 2jeb - SsExN-I (mutant) + ExN-II + RNA-binding protein 1
- 2hbj, 2hbk, 2hbl, 2hbm – yExN Rrp6 (mutant) - yeast
- 2ja9 – yExN Rrp40
- 2vnu – yExN Rrp44 + DNA
- 2wp8 - yExN Rrp45, Ski6, Dis3
- 2ioc - hExN Trex1 N terminal
- 2nn6 – hExN Rrp41, Rrp45, Rrp42, Rrp40, Prp43, Rrp46, Mtr3, Csl4, Rrp4
- 4wfc - yExN Rrp6 + Lrp1
- 4wfd - yExN Rrp6 + Lrp1 + Dob1
- 4ifd, 4oo1 - yExN Rrp6, Rrp45, Rrp42, Rrp40, Prp43, Rrp46, Mtr3, Csl4, Rrp4, Rrp6, Ski6, Dis3 + RNA
- Other ExN
- 1ir6, 2zxo, 2zxp, 2zxr – ExN Recj – Thermus thermophiles
- 1w0h – hExn Eri1 nuclease domain
- 1zbu - hExn Eri1
- 1zbh - hExn Eri1 (mutant) + DNA
- 1y97 – hExN Trex2
- 3zdk, 5aho – hExN Apollo
- 2o4g - mExN Trex1 + nucleotide – mouse
- 3mxj - mExN Trex1
- 2o4i, 3mxi, 3mxm, 3u3y, 3u6f - mExN Trex1 (mutant) + polynucleotide
- 2oa8 - mExN Trex1 + DNA
- 3b6o, 3b6p - mExN Trex1 + ion inhibitor
- 2w45 – H4ExN alkaline - Herpesvirus 4
- 2w4b - H4ExN alkaline (mutant)
- 3syy - LhExN alkaline – Laribacter hongkongensis
- 3sz4 - LhExN alkaline + AMP
- 3sz5 - LhExN alkaline + polynucleotide
- 3e2v – yExN
- 4lty, 4lu9, 4m0v – EcExN sbcD
ReferencesReferences
- ↑ Mukherjee D, Fritz DT, Kilpatrick WJ, Gao M, Wilusz J. Analysis of RNA exonucleolytic activities in cellular extracts. Methods Mol Biol. 2004;257:193-212. PMID:14770007 doi:http://dx.doi.org/10.1385/1-59259-750-5:193
- ↑ Breyer WA, Matthews BW. Structure of Escherichia coli exonuclease I suggests how processivity is achieved. Nat Struct Biol. 2000 Dec;7(12):1125-8. PMID:11101894 doi:10.1038/81978
