3d6u: Difference between revisions

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==Crystal structure of 4-(trifluoromethyldiazirinyl)phenylalanyl-tRNA synthetase==
==Crystal structure of 4-(trifluoromethyldiazirinyl)phenylalanyl-tRNA synthetase==
<StructureSection load='3d6u' size='340' side='right' caption='[[3d6u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3d6u' size='340' side='right' caption='[[3d6u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tyrS, MJ0389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 ATCC 43067])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tyrS, MJ0389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 ATCC 43067])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d6u OCA], [http://pdbe.org/3d6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d6u RCSB], [http://www.ebi.ac.uk/pdbsum/3d6u PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d6u OCA], [http://pdbe.org/3d6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d6u RCSB], [http://www.ebi.ac.uk/pdbsum/3d6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d6u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d6/3d6u_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d6/3d6u_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Aminoacyl-trna synthetase]]
[[Category: Aminoacyl-trna synthetase]]
[[Category: Atp-binding]]
[[Category: Atp-binding]]
[[Category: Cytoplasm]]
[[Category: Ligase]]
[[Category: Ligase]]
[[Category: Nucleotide-binding]]
[[Category: Nucleotide-binding]]
[[Category: Photocrosslinking unnatural amino acid]]
[[Category: Photocrosslinking unnatural amino acid]]
[[Category: Protein biosynthesis]]
[[Category: Protein biosynthesis]]

Revision as of 12:32, 14 November 2018

Crystal structure of 4-(trifluoromethyldiazirinyl)phenylalanyl-tRNA synthetaseCrystal structure of 4-(trifluoromethyldiazirinyl)phenylalanyl-tRNA synthetase

Structural highlights

3d6u is a 1 chain structure with sequence from Atcc 43067. This structure supersedes the now removed PDB entry 2q1g. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Gene:tyrS, MJ0389 (ATCC 43067)
Activity:Tyrosine--tRNA ligase, with EC number 6.1.1.1
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SYY_METJA] Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. Steer BA, Schimmel P. Major anticodon-binding region missing from an archaebacterial tRNA synthetase. J Biol Chem. 1999 Dec 10;274(50):35601-6. PMID:10585437

3d6u, resolution 2.20Å

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