4rtj: Difference between revisions
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==A non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and Sinefungin== | ==A non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and Sinefungin== | ||
<StructureSection load='4rtj' size='340' side='right' caption='[[4rtj]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='4rtj' size='340' side='right' caption='[[4rtj]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gip|2gip]], [[2ore|2ore]], [[4rtk|4rtk]], [[4rtl|4rtl]], [[4rtm|4rtm]], [[4rtn|4rtn]], [[4rto|4rto]], [[4rtp|4rtp]], [[4rtq|4rtq]], [[4rtr|4rtr]], [[4rts|4rts]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gip|2gip]], [[2ore|2ore]], [[4rtk|4rtk]], [[4rtl|4rtl]], [[4rtm|4rtm]], [[4rtn|4rtn]], [[4rto|4rto]], [[4rtp|4rtp]], [[4rtq|4rtq]], [[4rtr|4rtr]], [[4rts|4rts]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rtj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rtj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rtj RCSB], [http://www.ebi.ac.uk/pdbsum/4rtj PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rtj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rtj OCA], [http://pdbe.org/4rtj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rtj RCSB], [http://www.ebi.ac.uk/pdbsum/4rtj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rtj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4rtj" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[DNA adenine methylase|DNA adenine methylase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 04:55, 10 March 2017
A non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and SinefunginA non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and Sinefungin
Structural highlights
Publication Abstract from PubMedDNA adenine methyltransferase (Dam) is widespread and conserved among the gamma-proteobacteria. Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. Dam also controls virulence in many pathogenic Gram-negative bacteria. An unexplained and perplexing observation about Escherichia coli Dam (EcoDam) is that there is no obvious relationship between the genes that are transcriptionally responsive to Dam and the promoter-proximal presence of GATC sequences. Here, we demonstrate that EcoDam interacts with a 5-base pair non-cognate sequence distinct from GATC. The crystal structure of a non-cognate complex allowed us to identify a DNA binding element, GTYTA/TARAC (where Y = C/T and R = A/G). This element immediately flanks GATC sites in some Dam-regulated promoters, including the Pap operon which specifies pyelonephritis-associated pili. In addition, Dam interacts with near-cognate GATC sequences (i.e. 3/4-site ATC and GAT). Taken together, these results imply that Dam, in addition to being responsible for GATC methylation, could also function as a methylation-independent transcriptional repressor. Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.,Horton JR, Zhang X, Blumenthal RM, Cheng X Nucleic Acids Res. 2015 Apr 6. pii: gkv251. PMID:25845600[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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