2a6e: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2a6e |SIZE=350|CAPTION= <scene name='initialview01'>2a6e</scene>, resolution 2.80Å | |PDB= 2a6e |SIZE=350|CAPTION= <scene name='initialview01'>2a6e</scene>, resolution 2.80Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1iw7|1IW7]], [[1smy|1SMY]], [[2a68|2A68]], [[2a69|2A69]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6e OCA], [http://www.ebi.ac.uk/pdbsum/2a6e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a6e RCSB]</span> | |||
}} | }} | ||
| Line 34: | Line 37: | ||
[[Category: Vassylyeva, M N.]] | [[Category: Vassylyeva, M N.]] | ||
[[Category: Wakatsuki, S.]] | [[Category: Wakatsuki, S.]] | ||
[[Category: bridge helix]] | [[Category: bridge helix]] | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
| Line 43: | Line 44: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:26 2008'' | ||
Revision as of 01:48, 31 March 2008
| |||||||
| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1IW7, 1SMY, 2A68, 2A69
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the T. Thermophilus RNA polymerase holoenzyme
OverviewOverview
Rifamycins, the clinically important antibiotics, target bacterial RNA polymerase (RNAP). A proposed mechanism in which rifamycins sterically block the extension of nascent RNA beyond three nucleotides does not alone explain why certain RNAP mutations confer resistance to some but not other rifamycins. Here we show that unlike rifampicin and rifapentin, and contradictory to the steric model, rifabutin inhibits formation of the first and second phosphodiester bonds. We report 2.5 A resolution structures of rifabutin and rifapentin complexed with the Thermus thermophilus RNAP holoenzyme. The structures reveal functionally important distinct interactions of antibiotics with the initiation sigma factor. Strikingly, both complexes lack the catalytic Mg2+ ion observed in the apo-holoenzyme, whereas an increase in Mg2+ concentration confers resistance to rifamycins. We propose that a rifamycin-induced signal is transmitted over approximately 19 A to the RNAP active site to slow down catalysis. Based on structural predictions, we designed enzyme substitutions that apparently interrupt this allosteric signal.
About this StructureAbout this Structure
2A6E is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins., Artsimovitch I, Vassylyeva MN, Svetlov D, Svetlov V, Perederina A, Igarashi N, Matsugaki N, Wakatsuki S, Tahirov TH, Vassylyev DG, Cell. 2005 Aug 12;122(3):351-63. PMID:16096056
Page seeded by OCA on Mon Mar 31 01:48:26 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- DNA-directed RNA polymerase
- Protein complex
- Thermus thermophilus
- Artsimovitch, I.
- Igarashi, N.
- Matsugaki, N.
- Perederina, A.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Svetlov, D.
- Svetlov, V.
- Tahirov, T H.
- Vassylyev, D G.
- Vassylyeva, M N.
- Wakatsuki, S.
- Bridge helix
- Riken structural genomics/proteomics initiative
- Rna polymerase holoenzyme
- Rsgi
- Structural genomic
- Transcription regulation