4wf2: Difference between revisions

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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf2 PDBsum]</span></td></tr>
</table>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/BIRA_ECOLI BIRA_ECOLI]] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.<ref>PMID:6129246</ref> <ref>PMID:2667763</ref> <ref>PMID:8003500</ref> <ref>PMID:12527300</ref> 
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Beckett, D.]]
[[Category: Beckett, D]]
[[Category: Eginton, C.]]
[[Category: Eginton, C]]
[[Category: Wade, H.]]
[[Category: Wade, H]]
[[Category: Biotin protein ligase]]
[[Category: Biotin protein ligase]]
[[Category: Biotin repressor]]
[[Category: Biotin repressor]]
[[Category: Complex with biotinol-5'-amp]]
[[Category: Complex with biotinol-5'-amp]]
[[Category: G142a mutant]]
[[Category: G142a mutant]]

Revision as of 15:45, 25 December 2014

Structure of E. coli BirA G142A bound to biotinol-5'-AMPStructure of E. coli BirA G142A bound to biotinol-5'-AMP

Structural highlights

4wf2 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:[acetyl-CoA-carboxylase_ligase Biotin--[acetyl-CoA-carboxylase] ligase], with EC number 6.3.4.15
Resources:FirstGlance, OCA, RCSB, PDBsum

Function

[BIRA_ECOLI] Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.[1] [2] [3] [4]

References

  1. ↑ Eisenberg MA, Prakash O, Hsiung SC. Purification and properties of the biotin repressor. A bifunctional protein. J Biol Chem. 1982 Dec 25;257(24):15167-73. PMID:6129246
  2. ↑ Cronan JE Jr. The E. coli bio operon: transcriptional repression by an essential protein modification enzyme. Cell. 1989 Aug 11;58(3):427-9. PMID:2667763
  3. ↑ Xu Y, Beckett D. Kinetics of biotinyl-5'-adenylate synthesis catalyzed by the Escherichia coli repressor of biotin biosynthesis and the stability of the enzyme-product complex. Biochemistry. 1994 Jun 14;33(23):7354-60. PMID:8003500
  4. ↑ Streaker ED, Beckett D. Coupling of protein assembly and DNA binding: biotin repressor dimerization precedes biotin operator binding. J Mol Biol. 2003 Jan 31;325(5):937-48. PMID:12527300 doi:http://dx.doi.org/10.1016/S0022283602013086

4wf2, resolution 2.31Å

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