1x3e: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1x3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X3E FirstGlance]. <br> | <table><tr><td colspan='2'>[[1x3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X3E FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1x3f|1x3f]], [[1x3g|1x3g]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1x3f|1x3f]], [[1x3g|1x3g]]</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x3e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x3e RCSB], [http://www.ebi.ac.uk/pdbsum/1x3e PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x3e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x3e RCSB], [http://www.ebi.ac.uk/pdbsum/1x3e PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/SSB_MYCSM SSB_MYCSM]] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). Interacts with RecA. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 33: | Line 35: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Mycobacterium smegmatis]] | [[Category: Mycobacterium smegmatis]] | ||
[[Category: Dauter, Z | [[Category: Dauter, Z]] | ||
[[Category: Jeyakanthan, J | [[Category: Jeyakanthan, J]] | ||
[[Category: Manjunath, G P | [[Category: Manjunath, G P]] | ||
[[Category: Muniyappa, K | [[Category: Muniyappa, K]] | ||
[[Category: Saikrishnan, K | [[Category: Saikrishnan, K]] | ||
[[Category: Sekar, K | [[Category: Sekar, K]] | ||
[[Category: Singh, P | [[Category: Singh, P]] | ||
[[Category: Vijayan, M | [[Category: Vijayan, M]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Dna-binding protein]] | [[Category: Dna-binding protein]] | ||
[[Category: Oligonucleotide binding fold]] | [[Category: Oligonucleotide binding fold]] | ||
Revision as of 23:51, 24 December 2014
Crystal structure of the single-stranded DNA-binding protein from Mycobacterium smegmatisCrystal structure of the single-stranded DNA-binding protein from Mycobacterium smegmatis
Structural highlights
Function[SSB_MYCSM] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). Interacts with RecA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe structure of Mycobacterium smegmatis single-stranded DNA-binding protein (SSB) has been determined using three data sets collected from related crystals. The structure is similar to that of its homologue from Mycobacterium tuberculosis, indicating that the clamp arrangement that stabilizes the dimer and the ellipsoidal shape of the tetramer are characteristic features of mycobacterial SSBs. The central OB fold is conserved in mycobacterial SSBs as well as those from Escherichia coli, Deinococcus radiodurans and human mitochondria. However, the quaternary structure exhibits considerable variability. The observed plasticity of the subunit is related to this variability. The crystal structures and modelling provide a rationale for the variability. The strand involved in the clamp mechanism, which leads to higher stability of the tetramer, appears to occur in all high-G+C Gram-positive bacteria. The higher stability is perhaps required by these organisms. The mode of DNA binding of mycobacterial SSBs is different from that of E. coli SSB partly on account of the difference in the shape of the tetramers. Another difference between the two modes is that the former contains additional ionic interactions and is more susceptible to salt concentration. Structure of Mycobacterium smegmatis single-stranded DNA-binding protein and a comparative study involving homologus SSBs: biological implications of structural plasticity and variability in quaternary association.,Saikrishnan K, Manjunath GP, Singh P, Jeyakanthan J, Dauter Z, Sekar K, Muniyappa K, Vijayan M Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1140-8. Epub 2005, Jul 20. PMID:16041080[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||