2gwm: Difference between revisions

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Revision as of 18:10, 20 March 2008

File:2gwm.gif

, resolution 1.500Å

Ligands:

Gene:

mkaA (Salmonella typhimurium)

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of the Salmonella SpvB ATR Domain

OverviewOverview

Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.

About this StructureAbout this Structure

2GWM is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

ReferenceReference

A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:16905096

Page seeded by OCA on Thu Mar 20 17:10:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2gwm.gif|left|200px]]<br /><applet load="2gwm" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2gwm.gif|left|200px]]
-caption="2gwm, resolution 1.500&Aring;" />
-'''Crystal structure of the Salmonella SpvB ATR Domain'''<br />
+ {{Structure
+|PDB= 2gwm |SIZE=350|CAPTION= <scene name='initialview01'>2gwm</scene>, resolution 1.500&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE= mkaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
+}}
+'''Crystal structure of the Salmonella SpvB ATR Domain'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-GWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWM OCA].
+GWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWM OCA].
 ==Reference==
-A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16905096 16905096]
+A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905096 16905096]
 [[Category: Salmonella typhimurium]]
 [[Category: Single protein]]
@@ Line 20: / Line 29: @@
 [[Category: spvb]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:36:08 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:10:06 2008''