4a5u: Difference between revisions
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{{STRUCTURE_4a5u| PDB=4a5u | SCENE= }} | {{STRUCTURE_4a5u| PDB=4a5u | SCENE= }} | ||
===Turnip yellow mosaic virus proteinase and Escherichia coli 30S ribosomal S15=== | ===Turnip yellow mosaic virus proteinase and Escherichia coli 30S ribosomal S15=== | ||
{{ABSTRACT_PUBMED_23966860}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/POLR_TYMV POLR_TYMV]] Acts as a cysteine protease, methyltransferase and deubiquitinase. The cyteine protease activity cleaves the polyprotein giving rise to mature proteins. The methyltransferase domain is probably involved in viral RNA capping. The deubiquitylating activity counteracts the degradation of the viral polymerase mediated by the host ubiquitin-proteasome system. The polymerase is thus stabilized and infectivity is increased.<ref>PMID:22117220</ref> RNA-directed RNA polymerase is responsible for the replication and transcription of the genome.<ref>PMID:22117220</ref> [[http://www.uniprot.org/uniprot/E8Y371_ECOKO E8Y371_ECOKO]] Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity).[HAMAP-Rule:MF_01343] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA (By similarity).[HAMAP-Rule:MF_01343] | |||
==About this Structure== | ==About this Structure== | ||
[[4a5u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/ | [[4a5u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Tymv Tymv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5U OCA]. | ||
==See Also== | |||
*[[Ribosomal protein S15|Ribosomal protein S15]] | |||
==Reference== | |||
<ref group="xtra">PMID:023966860</ref><references group="xtra"/><references/> | |||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
[[Category: | [[Category: Tymv]] | ||
[[Category: Beaurepaire, L.]] | [[Category: Beaurepaire, L.]] | ||
[[Category: Bressanelli, S.]] | [[Category: Bressanelli, S.]] | ||
Revision as of 09:56, 13 November 2013
Turnip yellow mosaic virus proteinase and Escherichia coli 30S ribosomal S15Turnip yellow mosaic virus proteinase and Escherichia coli 30S ribosomal S15
Template:ABSTRACT PUBMED 23966860
FunctionFunction
[POLR_TYMV] Acts as a cysteine protease, methyltransferase and deubiquitinase. The cyteine protease activity cleaves the polyprotein giving rise to mature proteins. The methyltransferase domain is probably involved in viral RNA capping. The deubiquitylating activity counteracts the degradation of the viral polymerase mediated by the host ubiquitin-proteasome system. The polymerase is thus stabilized and infectivity is increased.[1] RNA-directed RNA polymerase is responsible for the replication and transcription of the genome.[2] [E8Y371_ECOKO] Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity).[HAMAP-Rule:MF_01343] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA (By similarity).[HAMAP-Rule:MF_01343]
About this StructureAbout this Structure
4a5u is a 2 chain structure with sequence from Escherichia coli and Tymv. Full crystallographic information is available from OCA.
See AlsoSee Also
ReferenceReference
- ↑ Lombardi C, Ayach M, Beaurepaire L, Chenon M, Andreani J, Guerois R, Jupin I, Bressanelli S. A compact viral processing proteinase/ubiquitin hydrolase from the OTU family. PLoS Pathog. 2013 Aug;9(8):e1003560. doi: 10.1371/journal.ppat.1003560. Epub 2013, Aug 15. PMID:23966860 doi:http://dx.doi.org/10.1371/journal.ppat.1003560
- ↑ Chenon M, Camborde L, Cheminant S, Jupin I. A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity. EMBO J. 2012 Feb 1;31(3):741-53. doi: 10.1038/emboj.2011.424. Epub 2011 Nov 25. PMID:22117220 doi:http://dx.doi.org/10.1038/emboj.2011.424
- ↑ Chenon M, Camborde L, Cheminant S, Jupin I. A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity. EMBO J. 2012 Feb 1;31(3):741-53. doi: 10.1038/emboj.2011.424. Epub 2011 Nov 25. PMID:22117220 doi:http://dx.doi.org/10.1038/emboj.2011.424