1hwq: Difference between revisions

Revision as of 12:42, 20 March 2008

Coordinates:

SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP

OverviewOverview

The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.

About this StructureAbout this Structure

1HWQ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168

Page seeded by OCA on Thu Mar 20 11:42:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hwq.gif|left|200px]]
-caption="1hwq" />
-'''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''<br />
+ {{Structure
+|PDB= 1hwq |SIZE=350|CAPTION= <scene name='initialview01'>1hwq</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].
+HWQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].
 ==Reference==
-A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11350168 11350168]
+A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11350168 11350168]
 [[Category: Protein complex]]
 [[Category: Dieckmann, T.]]
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 [[Category: vs ribozyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:42:29 2008''