1hwq

SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOPSOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP

Structural highlights 1hwq is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed. A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA.,Flinders J, Dieckmann T J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribozyme 3D structures

spinqualitylabelsall models 1hwq Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image