2igp: Difference between revisions

Revision as of 18:52, 21 February 2008

Crystal Structure of Hec1 CH domain

OverviewOverview

Kinetochores are multicomponent assemblies that connect chromosomal centromeres to mitotic-spindle microtubules. The Ndc80 complex is an essential core element of kinetochores, conserved from yeast to humans. It is a rod-like assembly of four proteins- Ndc80p (HEC1 in humans), Nuf2p, Spc24p and Spc25p. We describe here the crystal structure of the most conserved region of HEC1, which lies at one end of the rod and near the N terminus of the polypeptide chain. It folds into a calponin-homology domain, resembling the microtubule-binding domain of the plus-end-associated protein EB1. We show that an Ndc80p-Nuf2p heterodimer binds microtubules in vitro. The less conserved, N-terminal segment of Ndc80p contributes to the interaction and may be a crucial regulatory element. We propose that the Ndc80 complex forms a direct link between kinetochore core components and spindle microtubules.

About this StructureAbout this Structure

2IGP is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment., Wei RR, Al-Bassam J, Harrison SC, Nat Struct Mol Biol. 2007 Jan;14(1):54-9. Epub 2006 Dec 31. PMID:17195848

Page seeded by OCA on Thu Feb 21 17:52:29 2008

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OCA

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 ==Overview==
-Kinetochores are multicomponent assemblies that connect chromosomal, centromeres to mitotic-spindle microtubules. The Ndc80 complex is an, essential core element of kinetochores, conserved from yeast to humans. It, is a rod-like assembly of four proteins- Ndc80p (HEC1 in humans), Nuf2p, Spc24p and Spc25p. We describe here the crystal structure of the most, conserved region of HEC1, which lies at one end of the rod and near the N, terminus of the polypeptide chain. It folds into a calponin-homology, domain, resembling the microtubule-binding domain of the, plus-end-associated protein EB1. We show that an Ndc80p-Nuf2p heterodimer, binds microtubules in vitro. The less conserved, N-terminal segment of, Ndc80p contributes to the interaction and may be a crucial regulatory, element. We propose that the Ndc80 complex forms a direct link between, kinetochore core components and spindle microtubules.
+Kinetochores are multicomponent assemblies that connect chromosomal centromeres to mitotic-spindle microtubules. The Ndc80 complex is an essential core element of kinetochores, conserved from yeast to humans. It is a rod-like assembly of four proteins- Ndc80p (HEC1 in humans), Nuf2p, Spc24p and Spc25p. We describe here the crystal structure of the most conserved region of HEC1, which lies at one end of the rod and near the N terminus of the polypeptide chain. It folds into a calponin-homology domain, resembling the microtubule-binding domain of the plus-end-associated protein EB1. We show that an Ndc80p-Nuf2p heterodimer binds microtubules in vitro. The less conserved, N-terminal segment of Ndc80p contributes to the interaction and may be a crucial regulatory element. We propose that the Ndc80 complex forms a direct link between kinetochore core components and spindle microtubules.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Harrison, S.C.]]
+[[Category: Harrison, S C.]]
-[[Category: Wei, R.R.]]
+[[Category: Wei, R R.]]
 [[Category: BME]]
 [[Category: alpha helices]]
 [[Category: calponin homology (ch) domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:36:12 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:52:29 2008''