Sandbox Reserved 492: Difference between revisions

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OCA, Charlie Zogzas

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-== '''Uses & Potential Benefits''' ==
+== '''Studies & Potential Benefits''' ==
-While measures of treating someone with CX intoxication generally involves carefully replenishing electrolytes and other vital fluids there have been plenty of tests conducted that show that the toxin can be directly inhibited on the molecular level as well. The catalytic subunit of the CX protein has shown to bind, with high affinity a molecule known as PJ34- as well as other structurally conserved, fused-hydrocarbon ring inhibitors. Essentially, the <scene name='Sandbox_Reserved_492/Pj34_inhibitor/1'>PJ34</scene>replaces what would target protein in the epithelial cell of a human host and prevent the ill effects of intoxication...in theory.
+While CX intoxication from the bacterium is an incredibly serious and even life-threatening condition, tests conducted have shown that the toxin can be directly inhibited on the molecular level. The catalytic subunit of the CX protein is known to bind, with high affinity, to a molecule known as PJ34- as well as with other structurally conserved, fused-hydrocarbon ring inhibitors.[4] These types of interactions are studied through the use of computerized modeling, where factors such as Van der Waal radii, atomic radii, electron affinity etc.. are assessed producing viable molecular model.
-         {{clear}}     This is wheree the text would go
+                                          <Structure load='2Q6M' size='425' color='black' frame='true' align='middle' caption='X-Ray Diffraction image at 2.1Å of catalytic fragment with PJ34 inhibitor bound.' />
+Essentially, the <scene name='Sandbox_Reserved_492/Pj34_inhibitor/1'>PJ34</scene>replaces what would be the target protein in the epithelial cell of a human thus prevents the Cholera Toxin from interacting with its normal ligand; this would in turn prevent the ill effects of intoxication...in theory.
-<Structure load='2Q6M' size='425' color='black' frame='true' align='middle' caption='X-Ray Diffraction image at 2.1Å of catalytic fragment with PJ34 inhibitor bound.' />
+Cholix also serves several valuable purposes in Biological research. Dr. Pierre-Hervé Luppi and his laboratory recently discovered a new "tracing" method using cholera-toxin instead of the previously used classical molecules such as Horse Radish Peroxidase (HRP). In contrast to HRP, which is '''passively''' taken up by neurons, cholera-toxin (as we discussed previously) binds specifically to surface receptors of neurons and is can be '''actively''' taken up and transported by the axons. [**] This phenomenon helps enhance the sensitivity of cholera-toxin as a tracer, for studies dealing with neural function and the diseases known to affect it.
+{{clear}}
-An aspiration for research being done on the Cholera Toxin coincides with a current "hot topic" within the science community and society around the world: '''Stem Cell Research.''' There have been some recent findings indicating that the protein may be capable of interacting - regulation on the genetic level - some key factors in Neural Stem Cell '''(NSC)''' regeneration and differentiation. Known as Tie2, a membrane receptor, and Hes3 a transcription factor, these two indicators have been shown to directly interact with the Cholix Toxin. Moreover, there are even some implications that the protein, when combined with specific medium, boosted Stem Cell culture growth.<ref>[5]Androutsellis-Theotokis, Andreas, Stuart Walbridge, Deric M. Park, Russel R. Lonser, and Ronald D. McKay. “Cholera Toxin Regulates a Signaling Pathway Critical for the Expansion of Neural Stem Cell Cultures from the Fetal and Adult Rodent Brains.” PLoS ONE 5, (2010)</ref> Thus, we see that apart from its potential to cause human illness, CX also poses the potential to offer a solution to cancer and other related diseases. [[Image:NSC.jpeg]]
+As a result, aspirations for future research being done on Cholera Toxin today, coincide with a current "hot topic" within the science community and the entre World: '''Stem Cell Research.''' There have been some recent findings indicating that the protein may be capable of interacting - regulation on the genetic level - some key factors in Neural Stem Cell '''(NSC)''' regeneration and differentiation. Known as Tie2, a membrane receptor, and Hes3 a transcription factor, these two indicators have been shown to directly interact with the Cholix Toxin. Moreover, there are even some implications that the protein, when combined with specific medium, boosted Stem Cell culture growth.<ref>[5]Androutsellis-Theotokis, Andreas, Stuart Walbridge, Deric M. Park, Russel R. Lonser, and Ronald D. McKay. “Cholera Toxin Regulates a Signaling Pathway Critical for the Expansion of Neural Stem Cell Cultures from the Fetal and Adult Rodent Brains.” PLoS ONE 5, (2010)</ref> Thus, we see that apart from its potential to cause human illness, CX also poses the potential to offer a solution to cancer and other related diseases. [[Image:NSC.jpeg]]
 ==References ==
 <references/>