2q2e: Difference between revisions

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New page: left|200px<br /><applet load="2q2e" size="450" color="white" frame="true" align="right" spinBox="true" caption="2q2e, resolution 4.00Å" /> '''Crystal structure of...
 
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[[Image:2q2e.jpg|left|200px]]<br /><applet load="2q2e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2q2e, resolution 4.00&Aring;" />
'''Crystal structure of the topoisomerase VI holoenzyme from Methanosarcina mazei'''<br />


==Overview==
==Crystal structure of the topoisomerase VI holoenzyme from Methanosarcina mazei==
Type II topoisomerases help disentangle chromosomes to facilitate cell, division. To advance understanding of the structure and dynamics of these, essential enzymes, we have determined the crystal structure of an archaeal, type IIB topoisomerase, topo VI, at 4.0-A resolution. The 220-kDa, heterotetramer adopts a 'twin-gate' architecture, in which a pair of, ATPase domains at one end of the enzyme is poised to coordinate DNA, movements into the enzyme and through a set of DNA-cleaving domains at the, other end. Small-angle X-ray scattering studies show that nucleotide, binding elicits a major structural reorganization that is propagated to, the enzyme's DNA-cleavage center, explaining how ATP is coupled to DNA, capture and strand scission. These data afford important insights into the, mechanisms of topo VI and related proteins, including type IIA, topoisomerases and the Spo11 meiotic recombination endonuclease.
<StructureSection load='2q2e' size='340' side='right'caption='[[2q2e]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2q2e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q2E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q2e OCA], [https://pdbe.org/2q2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q2e RCSB], [https://www.ebi.ac.uk/pdbsum/2q2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q2e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TOP6A_METMA TOP6A_METMA] Relaxes both positive and negative superturns and exhibits a strong decatenase activity (By similarity).[HAMAP-Rule:MF_00132]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q2/2q2e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q2e ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2Q2E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2Q2E OCA].
*[[Topoisomerase 3D structures|Topoisomerase 3D structures]]
 
*[[WD-repeat protein 3D structures|WD-repeat protein 3D structures]]
==Reference==
__TOC__
Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI., Corbett KD, Benedetti P, Berger JM, Nat Struct Mol Biol. 2007 Jul;14(7):611-619. Epub 2007 Jul 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17603498 17603498]
</StructureSection>
[[Category: DNA topoisomerase (ATP-hydrolyzing)]]
[[Category: Large Structures]]
[[Category: Methanosarcina mazei]]
[[Category: Methanosarcina mazei]]
[[Category: Protein complex]]
[[Category: Benedetti P]]
[[Category: Benedetti, P.]]
[[Category: Berger JM]]
[[Category: Berger, J.M.]]
[[Category: Corbett KD]]
[[Category: Corbett, K.D.]]
[[Category: atpase]]
[[Category: dna-binding]]
[[Category: spo11]]
[[Category: topoisomerase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:45:42 2007''

Latest revision as of 12:13, 21 February 2024

Crystal structure of the topoisomerase VI holoenzyme from Methanosarcina mazeiCrystal structure of the topoisomerase VI holoenzyme from Methanosarcina mazei

Structural highlights

2q2e is a 2 chain structure with sequence from Methanosarcina mazei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TOP6A_METMA Relaxes both positive and negative superturns and exhibits a strong decatenase activity (By similarity).[HAMAP-Rule:MF_00132]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2q2e, resolution 4.00Å

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