1jfa: Difference between revisions

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New page: left|200px<br /><applet load="1jfa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jfa, resolution 2.5Å" /> '''TRICHODIENE SYNTHASE ...
 
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'''TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES'''<br />


==Overview==
==Trichodiene Synthase from Fusarium Sporotrichioides==
The x-ray crystal structure of recombinant trichodiene synthase from, Fusarium sporotrichioides has been determined to 2.5-A resolution, both, unliganded and complexed with inorganic pyrophosphate. This reaction, product coordinates to three Mg(2+) ions near the mouth of the active site, cleft. A comparison of the liganded and unliganded structures reveals a, ligand-induced conformational change that closes the mouth of the active, site cleft. Binding of the substrate farnesyl diphosphate similarly may, trigger this conformational change, which would facilitate catalysis by, protecting reactive carbocationic intermediates in the cyclization, cascade. Trichodiene synthase also shares significant structural, similarity with other sesquiterpene synthases despite a lack of, significant sequence identity. This similarity indicates divergence from a, common ancestor early in the evolution of terpene biosynthesis.
<StructureSection load='1jfa' size='340' side='right'caption='[[1jfa]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jfa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JFA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jfa OCA], [https://pdbe.org/1jfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jfa RCSB], [https://www.ebi.ac.uk/pdbsum/1jfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jfa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRI5_FUSSP TRI5_FUSSP] TS is a member of the terpene cyclase group of enzymes. It catalyzes the isomerization and cyclization of farnesyl pyro-phosphate to form trichodiene, the first cyclic intermediate in the biosynthetic pathway for trichothecenes. It serves to branch trichothecene biosynthesis from the isoprenoid pathway.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jfa_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jfa ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JFA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides] with EDO as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Trichodiene_synthase Trichodiene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.6 4.2.3.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JFA OCA].
*[[Trichodiene synthase|Trichodiene synthase]]
 
__TOC__
==Reference==
</StructureSection>
Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade., Rynkiewicz MJ, Cane DE, Christianson DW, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11698643 11698643]
[[Category: Fusarium sporotrichioides]]
[[Category: Fusarium sporotrichioides]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Trichodiene synthase]]
[[Category: Cane DE]]
[[Category: Cane, D.E.]]
[[Category: Christianson DW]]
[[Category: Christianson, D.W.]]
[[Category: Rynkiewicz MJ]]
[[Category: Rynkiewicz, M.J.]]
[[Category: EDO]]
[[Category: terpenoid synthase fold]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:11:21 2007''

Latest revision as of 10:39, 7 February 2024

Trichodiene Synthase from Fusarium SporotrichioidesTrichodiene Synthase from Fusarium Sporotrichioides

Structural highlights

1jfa is a 2 chain structure with sequence from Fusarium sporotrichioides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRI5_FUSSP TS is a member of the terpene cyclase group of enzymes. It catalyzes the isomerization and cyclization of farnesyl pyro-phosphate to form trichodiene, the first cyclic intermediate in the biosynthetic pathway for trichothecenes. It serves to branch trichothecene biosynthesis from the isoprenoid pathway.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1jfa, resolution 2.50Å

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