1gln: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1gln" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gln, resolution 2.5Å" /> '''ARCHITECTURES OF CLAS...
 
No edit summary
 
(18 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1gln.gif|left|200px]]<br /><applet load="1gln" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gln, resolution 2.5&Aring;" />
'''ARCHITECTURES OF CLASS-DEFINING AND SPECIFIC DOMAINS OF GLUTAMYL-TRNA SYNTHETASE'''<br />


==Overview==
==ARCHITECTURES OF CLASS-DEFINING AND SPECIFIC DOMAINS OF GLUTAMYL-TRNA SYNTHETASE==
The crystal structure of a class I aminoacyl-transfer RNA synthetase, glutamyl-tRNA synthetase (GluRS) from Thermus thermophilus, was solved and, refined at 2.5 A resolution. The amino-terminal half of GluRS shows a, geometrical similarity with that of Escherichia coli glutaminyl-tRNA, synthetase (GlnRS) of the same subclass in class I, comprising the class, I-specific Rossmann fold domain and the intervening subclass-specific, alpha/beta domain. These domains were found to have two GluRS-specific, secondary-structure insertions, which then participated in the specific, recognition of the D and acceptor stems of tRNA(Glu) as indicated by, mutagenesis analyses based on the docking properties of GluRS and tRNA. In, striking contrast to the beta-barrel structure of the GlnRS, carboxyl-terminal half, the GluRS carboxyl-terminal half displayed an, all-alpha-helix architecture, an alpha-helix cage, and mutagenesis, analyses indicated that it had a role in the anticodon recognition.
<StructureSection load='1gln' size='340' side='right'caption='[[1gln]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gln]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GLN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gln OCA], [https://pdbe.org/1gln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gln RCSB], [https://www.ebi.ac.uk/pdbsum/1gln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gln ProSAT], [https://www.topsan.org/Proteins/RSGI/1gln TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYE_THET8 SYE_THET8] Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).<ref>PMID:11224561</ref> <ref>PMID:17161369</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/1gln_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gln ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1GLN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GLN OCA].
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 
== References ==
==Reference==
<references/>
Architectures of class-defining and specific domains of glutamyl-tRNA synthetase., Nureki O, Vassylyev DG, Katayanagi K, Shimizu T, Sekine S, Kigawa T, Miyazawa T, Yokoyama S, Morikawa K, Science. 1995 Mar 31;267(5206):1958-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7701318 7701318]
__TOC__
[[Category: Single protein]]
</StructureSection>
[[Category: Thermus thermophilus]]
[[Category: Large Structures]]
[[Category: Katayanagi, K.]]
[[Category: Thermus thermophilus HB8]]
[[Category: Kigawa, T.]]
[[Category: Katayanagi K]]
[[Category: Miyazawa, T.]]
[[Category: Kigawa T]]
[[Category: Morikawa, K.]]
[[Category: Miyazawa T]]
[[Category: Nureki, O.]]
[[Category: Morikawa K]]
[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
[[Category: Nureki O]]
[[Category: Sekine, S.]]
[[Category: Sekine S]]
[[Category: Shimizu, T.]]
[[Category: Shimizu T]]
[[Category: Vassylyev, D.G.]]
[[Category: Vassylyev DG]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama S]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rsgi]]
[[Category: structural genomics]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:09:22 2007''

Latest revision as of 10:25, 7 February 2024

ARCHITECTURES OF CLASS-DEFINING AND SPECIFIC DOMAINS OF GLUTAMYL-TRNA SYNTHETASEARCHITECTURES OF CLASS-DEFINING AND SPECIFIC DOMAINS OF GLUTAMYL-TRNA SYNTHETASE

Structural highlights

1gln is a 1 chain structure with sequence from Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

SYE_THET8 Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S. Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase. Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:11224561 doi:10.1038/84927
  2. ↑ Sekine S, Shichiri M, Bernier S, Chenevert R, Lapointe J, Yokoyama S. Structural bases of transfer RNA-dependent amino acid recognition and activation by glutamyl-tRNA synthetase. Structure. 2006 Dec;14(12):1791-9. PMID:17161369 doi:10.1016/j.str.2006.10.005

1gln, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1gln&oldid=4044261"