1cyd: Difference between revisions

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New page: left|200px<br /><applet load="1cyd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cyd, resolution 1.8Å" /> '''CARBONYL REDUCTASE CO...
 
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[[Image:1cyd.gif|left|200px]]<br /><applet load="1cyd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cyd, resolution 1.8&Aring;" />
'''CARBONYL REDUCTASE COMPLEXED WITH NADPH AND 2-PROPANOL'''<br />


==Overview==
==CARBONYL REDUCTASE COMPLEXED WITH NADPH AND 2-PROPANOL==
BACKGROUND: Mouse lung carbonyl reductase (MLCR) is a member of the, short-chain dehydrogenase/reductase (SDR) family. Although it uses both, NADPH and NADH as coenzymes, the structural basis of its strong preference, for NADPH is unknown. RESULTS: The crystal structure of the ternary, complex of MLCR (with NADPH and 2-propanol) has been determined at 1.8 A, resolution. This is the first three-dimensional structure of a carbonyl, reductase, and MLCR is the first member of the SDR family to be solved in, complex with NADPH (rather than NADH). Comparison of the MLCR ternary, complex with three structures reported previously for enzymes of the SDR, family (all crystallized as complexes with NADH) reveals a pair of basic, residues (Lys17 and Arg39) making strong electrostatic interactions with, the 2'-phosphate group of NADPH. This pair of residues is well conserved, among the NADPH-preferring enzymes of the SDR family, but not among the, NADH-preferring enzymes. In the latter, an aspartate side chain occupies, the position of the two basic side chains. The aspartate residue, which, would come into unacceptably close contact with the 2'-phosphate group of, the adenosine moiety of NADPH, is replaced by a threonine or alanine in, the primary sequences of NADPH-preferring enzymes of the SDR family., CONCLUSIONS: The cofactor preferences exhibited by the enzymes of the SDR, family are mainly determined by the electrostatic environment surrounding, the 2'-hydroxyl (or phosphate) group of the adenosine ribose moiety of, NADH (or NADPH). Thus, positively charged and negatively charged, environments correlate with preference for NADPH and NADH respectively.
<StructureSection load='1cyd' size='340' side='right'caption='[[1cyd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cyd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CYD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyd OCA], [https://pdbe.org/1cyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cyd RCSB], [https://www.ebi.ac.uk/pdbsum/1cyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cyd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CBR2_MOUSE CBR2_MOUSE] May function in the pulmonary metabolism of endogenous carbonyl compounds, such as aliphatic aldehydes and ketones derived from lipid peroxidation, 3-ketosteroids and fatty aldehydes, as well as in xenobiotic metabolism.<ref>PMID:7705352</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cy/1cyd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cyd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CYD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAP and IPA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CYD OCA].
*[[Carbonyl reductase|Carbonyl reductase]]
 
*[[Carbonyl reductase 3D structures|Carbonyl reductase 3D structures]]
==Reference==
== References ==
Crystal structure of the ternary complex of mouse lung carbonyl reductase at 1.8 A resolution: the structural origin of coenzyme specificity in the short-chain dehydrogenase/reductase family., Tanaka N, Nonaka T, Nakanishi M, Deyashiki Y, Hara A, Mitsui Y, Structure. 1996 Jan 15;4(1):33-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8805511 8805511]
<references/>
[[Category: Carbonyl reductase (NADPH)]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Mitsui Y]]
[[Category: Mitsui, Y.]]
[[Category: Nonaka T]]
[[Category: Nonaka, T.]]
[[Category: Tanaka N]]
[[Category: Tanaka, N.]]
[[Category: IPA]]
[[Category: NAP]]
[[Category: oxidoreductase]]
[[Category: short-chain dehydrogenase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:50:55 2007''

Latest revision as of 09:46, 7 February 2024

CARBONYL REDUCTASE COMPLEXED WITH NADPH AND 2-PROPANOLCARBONYL REDUCTASE COMPLEXED WITH NADPH AND 2-PROPANOL

Structural highlights

1cyd is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CBR2_MOUSE May function in the pulmonary metabolism of endogenous carbonyl compounds, such as aliphatic aldehydes and ketones derived from lipid peroxidation, 3-ketosteroids and fatty aldehydes, as well as in xenobiotic metabolism.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Nakanishi M, Deyashiki Y, Ohshima K, Hara A. Cloning, expression and tissue distribution of mouse tetrameric carbonyl reductase. Identity with an adipocyte 27-kDa protein. Eur J Biochem. 1995 Mar 1;228(2):381-7. PMID:7705352

1cyd, resolution 1.80Å

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