2rhs: Difference between revisions

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[[Image:2rhs.jpg|left|200px]]


<!--
==PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies==
The line below this paragraph, containing "STRUCTURE_2rhs", creates the "Structure Box" on the page.
<StructureSection load='2rhs' size='340' side='right'caption='[[2rhs]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2rhs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_haemolyticus Staphylococcus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RHS FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAX:1-{3-[(4-PYRIDIN-2-YLPIPERAZIN-1-YL)SULFONYL]PHENYL}-3-(1,3-THIAZOL-2-YL)UREA'>GAX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
{{STRUCTURE_2rhs| PDB=2rhs |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rhs OCA], [https://pdbe.org/2rhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rhs RCSB], [https://www.ebi.ac.uk/pdbsum/2rhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rhs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYFA_STAHJ SYFA_STAHJ]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/2rhs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rhs ConSurf].
<div style="clear:both"></div>


'''PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies'''
==See Also==
 
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
In this article, we describe for the first time the high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus. We demonstrate the subtle yet important structural differences between this enzyme and the previously described Thermus thermophilus ortholog. We also explain the structure-activity relationship of several recently reported inhibitors. The native enzyme crystals were of poor quality--they only diffracted X-rays to 3-5A resolution. Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. This methodology is discussed and contrasted with the more traditional domain truncation approach.
[[Category: Large Structures]]
 
==About this Structure==
2RHS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_haemolyticus Staphylococcus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RHS OCA].
 
==Reference==
Rational protein engineering in action: the first crystal structure of a phenylalanine tRNA synthetase from Staphylococcus haemolyticus., Evdokimov AG, Mekel M, Hutchings K, Narasimhan L, Holler T, McGrath T, Beattie B, Fauman E, Yan C, Heaslet H, Walter R, Finzel B, Ohren J, McConnell P, Braden T, Sun F, Spessard C, Banotai C, Al-Kassim L, Ma W, Wengender P, Kole D, Garceau N, Toogood P, Liu J, J Struct Biol. 2008 Apr;162(1):152-69. Epub 2007 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18086534 18086534]
[[Category: Phenylalanine--tRNA ligase]]
[[Category: Protein complex]]
[[Category: Staphylococcus haemolyticus]]
[[Category: Staphylococcus haemolyticus]]
[[Category: Evdokimov, A G.]]
[[Category: Evdokimov AG]]
[[Category: Mekel, M.]]
[[Category: Mekel M]]
[[Category: Aminoacyl-trna synthetase]]
[[Category: Atp-binding]]
[[Category: Cytoplasm]]
[[Category: Heterotetramer]]
[[Category: Ligase]]
[[Category: Magnesium]]
[[Category: Metal-binding]]
[[Category: Nucleotide-binding]]
[[Category: Phenylalanine]]
[[Category: Protein biosynthesis]]
[[Category: Rna-binding]]
[[Category: Trna]]
[[Category: Trna-binding]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May  7 08:51:26 2008''

Latest revision as of 12:22, 21 February 2024

PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studiesPheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies

Structural highlights

2rhs is a 4 chain structure with sequence from Staphylococcus haemolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SYFA_STAHJ

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2rhs, resolution 2.20Å

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OCA
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