2kin: Difference between revisions

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New page: left|200px<br /> <applet load="2kin" size="450" color="white" frame="true" align="right" spinBox="true" caption="2kin, resolution 2.Å" /> '''KINESIN (MONOMERIC) F...
 
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[[Image:2kin.gif|left|200px]]<br />
<applet load="2kin" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2kin, resolution 2.&Aring;" />
'''KINESIN (MONOMERIC) FROM RATTUS NORVEGICUS'''<br />


==Overview==
==KINESIN (MONOMERIC) FROM RATTUS NORVEGICUS==
We have determined the X-ray structure of rat kinesin head and neck, domains. The folding of the core motor domain resembles that of human, kinesin reported recently [Kull, F. J., et al. (1996) Nature 380, 550-554]. Novel features of the structure include the N-terminal region, folded as a beta-strand, and the C-terminal transition from the motor to, the rod domain, folded as two beta-strands plus an alpha-helix. This helix, is the beginning of kinesin's neck responsible for dimerization of the, motor complex and for force transduction. Although the folding of the, motor domain core is similar to that of a domain of myosin (an, actin-dependent motor), the position and angle of kinesin's neck are very, different from those of myosin's stalk, suggesting that the two motors, have different mechanisms of force transduction. The N- and C-terminal, ends of the core motor, thought to be responsible for the directionality, of the motors [Case, R. B., et al. (1997) Cell 90, 959-966], take the form, of beta-strands attached to the central beta-sheet of the structure.
<StructureSection load='2kin' size='340' side='right'caption='[[2kin]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2kin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The April 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Kinesin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_4 10.2210/rcsb_pdb/mom_2005_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KIN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kin OCA], [https://pdbe.org/2kin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kin RCSB], [https://www.ebi.ac.uk/pdbsum/2kin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kin ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KIF5C_RAT KIF5C_RAT] Microtubule-associated force-producing protein that may play a role in organelle transport (By similarity). Has ATPase activity (PubMed:27452403). Involved in synaptic transmission (By similarity). Mediates dendritic trafficking of mRNAs (By similarity). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (PubMed:23576431).[UniProtKB:O60282][UniProtKB:P28738]<ref>PMID:23576431</ref> <ref>PMID:27452403</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/2kin_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kin ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2KIN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SO4 and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2KIN with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb64_1.html Kinesin]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2KIN OCA].
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
 
== References ==
==Reference==
<references/>
X-ray structure of motor and neck domains from rat brain kinesin., Sack S, Muller J, Marx A, Thormahlen M, Mandelkow EM, Brady ST, Mandelkow E, Biochemistry. 1997 Dec 23;36(51):16155-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9405049 9405049]
__TOC__
</StructureSection>
[[Category: Kinesin]]
[[Category: Kinesin]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Biou, V.]]
[[Category: Biou V]]
[[Category: Brady, S.T.]]
[[Category: Brady ST]]
[[Category: Kozielski, F.]]
[[Category: Kozielski F]]
[[Category: Mandelkow, E.]]
[[Category: Mandelkow E]]
[[Category: Mandelkow, E.M.]]
[[Category: Mandelkow E-M]]
[[Category: Marx, A.]]
[[Category: Marx A]]
[[Category: Muller, J.]]
[[Category: Muller J]]
[[Category: Sack, S.]]
[[Category: Sack S]]
[[Category: Thormahlen, M.]]
[[Category: Thormahlen M]]
[[Category: ADP]]
[[Category: SO4]]
[[Category: cytoskeleton]]
[[Category: motor protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:08:59 2007''

Latest revision as of 12:04, 21 February 2024

KINESIN (MONOMERIC) FROM RATTUS NORVEGICUSKINESIN (MONOMERIC) FROM RATTUS NORVEGICUS

Structural highlights

2kin is a 2 chain structure with sequence from Rattus norvegicus. The April 2005 RCSB PDB Molecule of the Month feature on Kinesin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KIF5C_RAT Microtubule-associated force-producing protein that may play a role in organelle transport (By similarity). Has ATPase activity (PubMed:27452403). Involved in synaptic transmission (By similarity). Mediates dendritic trafficking of mRNAs (By similarity). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (PubMed:23576431).[UniProtKB:O60282][UniProtKB:P28738][1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Sun T, Yu N, Zhai LK, Li N, Zhang C, Zhou L, Huang Z, Jiang XY, Shen Y, Chen ZY. c-Jun NH2-terminal kinase (JNK)-interacting protein-3 (JIP3) regulates neuronal J Biol Chem. 2013 May 17;288(20):14531-14543. PMID:23576431 doi:10.1074/jbc.M113.464453
  2. ↑ Yamagishi M, Shigematsu H, Yokoyama T, Kikkawa M, Sugawa M, Aoki M, Shirouzu M, Yajima J, Nitta R. Structural Basis of Backwards Motion in Kinesin-1-Kinesin-14 Chimera: Implication for Kinesin-14 Motility. Structure. 2016 Aug 2;24(8):1322-34. doi: 10.1016/j.str.2016.05.021. Epub 2016, Jul 21. PMID:27452403 doi:http://dx.doi.org/10.1016/j.str.2016.05.021

2kin, resolution 2.00Å

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