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==== | ==cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex class1== | ||
<StructureSection load='7udc' size='340' side='right'caption='[[7udc]]' scene=''> | <StructureSection load='7udc' size='340' side='right'caption='[[7udc]], [[Resolution|resolution]] 3.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7udc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UDC FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7udc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7udc OCA], [https://pdbe.org/7udc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7udc RCSB], [https://www.ebi.ac.uk/pdbsum/7udc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7udc ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7udc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7udc OCA], [https://pdbe.org/7udc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7udc RCSB], [https://www.ebi.ac.uk/pdbsum/7udc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7udc ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Munc18-1 forms a template to organize assembly of the neuronal SNARE complex that triggers neurotransmitter release, binding first to a closed conformation of syntaxin-1 where its amino-terminal region interacts with the SNARE motif, and later binding to synaptobrevin. However, the mechanism of SNARE complex assembly remains unclear. Here, we report two cryo-EM structures of Munc18-1 bound to cross-linked syntaxin-1 and synaptobrevin. The structures allow visualization of how syntaxin-1 opens and reveal how part of the syntaxin-1 amino-terminal region can help nucleate interactions between the amino termini of the syntaxin-1 and synaptobrevin SNARE motifs, while their carboxyl termini bind to distal sites of Munc18-1. These observations, together with mutagenesis, SNARE complex assembly experiments, and fusion assays with reconstituted proteoliposomes, support a model whereby these interactions are critical to initiate SNARE complex assembly and multiple energy barriers enable diverse mechanisms for exquisite regulation of neurotransmitter release. | |||
SNARE assembly enlightened by cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex.,Stepien KP, Xu J, Zhang X, Bai XC, Rizo J Sci Adv. 2022 Jun 24;8(25):eabo5272. doi: 10.1126/sciadv.abo5272. Epub 2022 Jun , 22. PMID:35731863<ref>PMID:35731863</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7udc" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Syntaxin 3D structures|Syntaxin 3D structures]] | |||
*[[Syntaxin-binding protein|Syntaxin-binding protein]] | |||
*[[Vesicle-associated membrane protein 3D structures|Vesicle-associated membrane protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Rattus norvegicus]] | ||
[[Category: Bai X]] | |||
[[Category: Rizo J]] | |||
[[Category: Stepien KP]] | |||
[[Category: Xu J]] | |||
[[Category: Zhang X]] | |||
Latest revision as of 12:14, 17 October 2024
cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex class1cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex class1
Structural highlights
Publication Abstract from PubMedMunc18-1 forms a template to organize assembly of the neuronal SNARE complex that triggers neurotransmitter release, binding first to a closed conformation of syntaxin-1 where its amino-terminal region interacts with the SNARE motif, and later binding to synaptobrevin. However, the mechanism of SNARE complex assembly remains unclear. Here, we report two cryo-EM structures of Munc18-1 bound to cross-linked syntaxin-1 and synaptobrevin. The structures allow visualization of how syntaxin-1 opens and reveal how part of the syntaxin-1 amino-terminal region can help nucleate interactions between the amino termini of the syntaxin-1 and synaptobrevin SNARE motifs, while their carboxyl termini bind to distal sites of Munc18-1. These observations, together with mutagenesis, SNARE complex assembly experiments, and fusion assays with reconstituted proteoliposomes, support a model whereby these interactions are critical to initiate SNARE complex assembly and multiple energy barriers enable diverse mechanisms for exquisite regulation of neurotransmitter release. SNARE assembly enlightened by cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex.,Stepien KP, Xu J, Zhang X, Bai XC, Rizo J Sci Adv. 2022 Jun 24;8(25):eabo5272. doi: 10.1126/sciadv.abo5272. Epub 2022 Jun , 22. PMID:35731863[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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