6d8m: Difference between revisions
New page: '''Unreleased structure''' The entry 6d8m is ON HOLD Authors: Shoffner, G.M. Description: Group I self-splicing intron P4-P6 domain mutant A125U/G126U [[Category: Unreleased Structures... |
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==Group I self-splicing intron P4-P6 domain mutant A125U/G126U== | |||
<StructureSection load='6d8m' size='340' side='right'caption='[[6d8m]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6d8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D8M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8m OCA], [https://pdbe.org/6d8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d8m RCSB], [https://www.ebi.ac.uk/pdbsum/6d8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8m ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Crystallography is a major technique for determining large RNA structures. Obtaining diffraction-quality crystals has been the bottleneck. Although several RNA crystallization methods have been developed, the field strongly needs additional approaches. Here we invented an in crystallo selection strategy for identifying mutations that enhance a target RNA's crystallizability. The strategy includes constructing an RNA pool containing random mutations, obtaining crystals, and amplifying the sequences enriched by crystallization. We demonstrated a proof-of-principle application to the P4-P6 domain from the Tetrahymena ribozyme. We further determined the structures of four selected mutants. All four establish new crystal lattice contacts while maintaining the native structure. Three mutants achieve this by relocating bulges and one by making a helix more flexible. In crystallo selection provides opportunities to improve crystals of RNAs or RNA-ligand complexes. Our results also suggest that mutants may be rationally designed for crystallization by "walking" a bulge along the RNA chain. | |||
In Crystallo Selection to Establish New RNA Crystal Contacts.,Shoffner GM, Wang R, Podell E, Cech TR, Guo F Structure. 2018 May 28. pii: S0969-2126(18)30169-2. doi:, 10.1016/j.str.2018.05.005. PMID:29910185<ref>PMID:29910185</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Shoffner | <div class="pdbe-citations 6d8m" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Ribozyme 3D structures|Ribozyme 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tetrahymena thermophila]] | |||
[[Category: Shoffner GM]] | |||