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==Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4== | ==Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4== | ||
<StructureSection load='1vfz' size='340' side='right' caption='[[1vfz]], [[Resolution|resolution]] 2.24Å' scene=''> | <StructureSection load='1vfz' size='340' side='right'caption='[[1vfz]], [[Resolution|resolution]] 2.24Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1vfz]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1vfz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VFZ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vfz OCA], [https://pdbe.org/1vfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vfz RCSB], [https://www.ebi.ac.uk/pdbsum/1vfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vfz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/KIF1A_MOUSE KIF1A_MOUSE] Motor for anterograde axonal transport of synaptic vesicle precursors. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/1vfz_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/1vfz_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 29: | Line 29: | ||
</div> | </div> | ||
<div class="pdbe-citations 1vfz" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1vfz" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Kinesin 3D Structures|Kinesin 3D Structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Hirokawa | [[Category: Mus musculus]] | ||
[[Category: Kikkawa | [[Category: Hirokawa N]] | ||
[[Category: Nitta | [[Category: Kikkawa M]] | ||
[[Category: Okada | [[Category: Nitta R]] | ||
[[Category: Okada Y]] | |||
Latest revision as of 10:53, 25 October 2023
Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4
Structural highlights
FunctionKIF1A_MOUSE Motor for anterograde axonal transport of synaptic vesicle precursors. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe motor protein kinesin moves along microtubules, driven by adenosine triphosphate (ATP) hydrolysis. However, it remains unclear how kinesin converts the chemical energy into mechanical movement. We report crystal structures of monomeric kinesin KIF1A with three transition-state analogs: adenylyl imidodiphosphate (AMP-PNP), adenosine diphosphate (ADP)-vanadate, and ADP-AlFx (aluminofluoride complexes). These structures, together with known structures of the ADP-bound state and the adenylyl-(beta,gamma-methylene) diphosphate (AMP-PCP)-bound state, show that kinesin uses two microtubule-binding loops in an alternating manner to change its interaction with microtubules during the ATP hydrolysis cycle; loop L11 is extended in the AMP-PNP structure, whereas loop L12 is extended in the ADP structure. ADP-vanadate displays an intermediate structure in which a conformational change in two switch regions causes both loops to be raised from the microtubule, thus actively detaching kinesin. KIF1A alternately uses two loops to bind microtubules.,Nitta R, Kikkawa M, Okada Y, Hirokawa N Science. 2004 Jul 30;305(5684):678-83. PMID:15286375[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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