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==Crystal structure of the domain swapped PXB/TPR domain of mouse SNX21==
==Crystal structure of the domain swapped PXB/TPR domain of mouse SNX21==
<StructureSection load='4ymr' size='340' side='right' caption='[[4ymr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='4ymr' size='340' side='right'caption='[[4ymr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ymr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YMR FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ymr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YMR FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ymr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ymr OCA], [http://pdbe.org/4ymr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ymr RCSB], [http://www.ebi.ac.uk/pdbsum/4ymr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ymr ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ymr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ymr OCA], [https://pdbe.org/4ymr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ymr RCSB], [https://www.ebi.ac.uk/pdbsum/4ymr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ymr ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/SNX21_MOUSE SNX21_MOUSE] Binds to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(P3)) and phosphatidylinositol 4,5-bisphosphate (PubMed:25882846). May be involved in several stages of intracellular trafficking.<ref>PMID:25882846</ref>  
Sorting nexins (SNX) orchestrate membrane trafficking and signalling events required for the proper distribution of proteins within the endosomal network. Their phox homology (PX) domain acts as a phosphinositide (PI) recognition module that targets them to specific endocytic membrane domains. The modularity of SNX proteins confers a wide variety of functions from signalling to membrane deformation and cargo binding, and many SNXs are crucial modulators of endosome dynamics and are involved in a myriad of physiological and pathological processes such as neurodegenerative diseases, cancer and inflammation. Here, we have studied the poorly characterized SNX20 and its paralogue SNX21, which contain an N-terminal PX domain and a C-terminal PX-associated B (PXB) domain of unknown function. The two proteins share similar PI-binding properties and are recruited to early endosomal compartments by their PX domain. The crystal structure of the SNX21 PXB domain reveals a tetratricopeptide repeat (TPR) fold, a module that typically binds short peptide motifs, with three TPR alpha-helical repeats. However, the C-terminal capping helix adopts a highly unusual and potentially self-inhibitory topology. SAXS solution structures of SNX20 and SNX21 show that these proteins adopt a compact globular architecture, and membrane interaction analyses indicate the presence of overlapping PI-binding sites that may regulate their intracellular localisation. This study provides the first structural analysis of this poorly characterized subfamily of SNX proteins, highlighting a likely role as endosome-associated scaffolds.
 
Structure and membrane binding properties of the endosomal tetratricopeptide repeat (TPR) domain-containing sorting nexins SNX20 and SNX21.,Clairfeuille T, Norwood SJ, Qi X, Teasdale RD, Collins BM J Biol Chem. 2015 Apr 16. pii: jbc.M115.650598. PMID:25882846<ref>PMID:25882846</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ymr" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Sorting nexin|Sorting nexin]]
*[[Sorting nexin 3D structures|Sorting nexin 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Clairfeuille, T]]
[[Category: Large Structures]]
[[Category: Collins, B C]]
[[Category: Mus musculus]]
[[Category: Teasdale, R D]]
[[Category: Clairfeuille T]]
[[Category: Tetratricopeptide repeat endosome trafficking]]
[[Category: Collins BC]]
[[Category: Teasdale RD]]

Latest revision as of 16:04, 1 March 2024

Crystal structure of the domain swapped PXB/TPR domain of mouse SNX21Crystal structure of the domain swapped PXB/TPR domain of mouse SNX21

Structural highlights

4ymr is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SNX21_MOUSE Binds to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(P3)) and phosphatidylinositol 4,5-bisphosphate (PubMed:25882846). May be involved in several stages of intracellular trafficking.[1]

See Also

References

  1. ↑ Clairfeuille T, Norwood SJ, Qi X, Teasdale RD, Collins BM. Structure and membrane binding properties of the endosomal tetratricopeptide repeat (TPR) domain-containing sorting nexins SNX20 and SNX21. J Biol Chem. 2015 Apr 16. pii: jbc.M115.650598. PMID:25882846 doi:http://dx.doi.org/10.1074/jbc.M115.650598

4ymr, resolution 2.40Å

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