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==Crystal structure of ArnB aminotransferase with pyridomine 5' phosphate== | ==Crystal structure of ArnB aminotransferase with pyridomine 5' phosphate== | ||
<StructureSection load='1mdo' size='340' side='right' caption='[[1mdo]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='1mdo' size='340' side='right'caption='[[1mdo]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1mdo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MDO OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[1mdo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MDO FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mdo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mdo OCA], [https://pdbe.org/1mdo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mdo RCSB], [https://www.ebi.ac.uk/pdbsum/1mdo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mdo ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1mdo_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1mdo_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| Line 31: | Line 29: | ||
==See Also== | ==See Also== | ||
*[[Aminotransferase|Aminotransferase]] | *[[Aminotransferase 3D structures|Aminotransferase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Badger | [[Category: Large Structures]] | ||
[[Category: Buchanan | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] | ||
[[Category: Buchanan | [[Category: Badger J]] | ||
[[Category: Christopher | [[Category: Buchanan MD]] | ||
[[Category: Gajiwala | [[Category: Buchanan SG]] | ||
[[Category: Hendle | [[Category: Christopher JA]] | ||
[[Category: Muller-Dieckmann | [[Category: Gajiwala K]] | ||
[[Category: Newman | [[Category: Hendle J]] | ||
[[Category: Noland | [[Category: Muller-Dieckmann H-J]] | ||
[[Category: Rutter | [[Category: Newman JM]] | ||
[[Category: Sanderson | [[Category: Noland BW]] | ||
[[Category: | [[Category: Rutter ME]] | ||
[[Category: | [[Category: Sanderson WE]] | ||
[[Category: | [[Category: Sauder JM]] | ||
[[Category: Tresser J]] | |||
[[Category: Wright T]] | |||
Latest revision as of 03:14, 21 November 2024
Crystal structure of ArnB aminotransferase with pyridomine 5' phosphateCrystal structure of ArnB aminotransferase with pyridomine 5' phosphate
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedLipid A modification with 4-amino-4-deoxy-L-arabinose confers on certain pathogenic bacteria, such as Salmonella, resistance to cationic antimicrobial peptides, including those derived from the innate immune system. ArnB catalysis of amino group transfer from glutamic acid to the 4"-position of a UDP-linked ketopyranose molecule to form UDP-4-amino-4-deoxy-L-arabinose represents a key step in the lipid A modification pathway. Structural and functional studies of the ArnB aminotransferase were undertaken by combining X-ray crystallography with biochemical analyses. High-resolution crystal structures were solved for two native forms and one covalently inhibited form of S. typhimurium ArnB. These structures permitted identification of key residues involved in substrate binding and catalysis, including a rarely observed nonprolyl cis peptide bond in the active site. Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme.,Noland BW, Newman JM, Hendle J, Badger J, Christopher JA, Tresser J, Buchanan MD, Wright TA, Rutter ME, Sanderson WE, Muller-Dieckmann HJ, Gajiwala KS, Buchanan SG Structure. 2002 Nov;10(11):1569-80. PMID:12429098[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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