4yd9: Difference between revisions
New page: '''Unreleased structure''' The entry 4yd9 is ON HOLD Authors: Matsuno, A., Gai, Z., Kato, K., Tanaka, Y., Yao, M. Description: Category: Unreleased Structures Category: Gai, Z... |
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==Crystal structure of squid hemocyanin== | |||
<StructureSection load='4yd9' size='340' side='right'caption='[[4yd9]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4yd9]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Todarodes_pacificus Todarodes pacificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YD9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CUO:CU2-O2+CLUSTER'>CUO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yd9 OCA], [https://pdbe.org/4yd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yd9 RCSB], [https://www.ebi.ac.uk/pdbsum/4yd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yd9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Molluscan hemocyanin, a copper-containing oxygen transporter, is one of the largest known proteins. Although molluscan hemocyanins are currently applied as immunotherapeutic agents, their precise structure has not been determined because of their enormous size. Here, we have determined the first X-ray crystal structure of intact molluscan hemocyanin. The structure unveiled the architecture of the 3.8-MDa supermolecule composed of homologous functional units (FUs), wherein the dimers of FUs hierarchically associated to form the entire cylindrical decamer. Most of the specific inter-FU interactions were localized at narrow regions in the FU dimers, suggesting that rigid FU dimers formed by specific interactions assemble with flexibility. Furthermore, the roles of carbohydrates in assembly and allosteric effect, and conserved sulfur-containing residues in copper incorporation, were revealed. The precise structural information obtained in this study will accelerate our understanding of the molecular basis of hemocyanin and its future applications. | |||
Crystal Structure of the 3.8-MDa Respiratory Supermolecule Hemocyanin at 3.0 A Resolution.,Gai Z, Matsuno A, Kato K, Kato S, Khan MR, Shimizu T, Yoshioka T, Kato Y, Kishimura H, Kanno G, Miyabe Y, Terada T, Tanaka Y, Yao M Structure. 2015 Dec 1;23(12):2204-12. doi: 10.1016/j.str.2015.09.008. Epub 2015, Oct 22. PMID:26602184<ref>PMID:26602184</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4yd9" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Kato | <references/> | ||
[[Category: Matsuno | __TOC__ | ||
[[Category: Tanaka | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Todarodes pacificus]] | |||
[[Category: Gai Z]] | |||
[[Category: Kato K]] | |||
[[Category: Matsuno A]] | |||
[[Category: Tanaka Y]] | |||
[[Category: Yao M]] | |||
Latest revision as of 18:34, 8 November 2023
Crystal structure of squid hemocyaninCrystal structure of squid hemocyanin
Structural highlights
Publication Abstract from PubMedMolluscan hemocyanin, a copper-containing oxygen transporter, is one of the largest known proteins. Although molluscan hemocyanins are currently applied as immunotherapeutic agents, their precise structure has not been determined because of their enormous size. Here, we have determined the first X-ray crystal structure of intact molluscan hemocyanin. The structure unveiled the architecture of the 3.8-MDa supermolecule composed of homologous functional units (FUs), wherein the dimers of FUs hierarchically associated to form the entire cylindrical decamer. Most of the specific inter-FU interactions were localized at narrow regions in the FU dimers, suggesting that rigid FU dimers formed by specific interactions assemble with flexibility. Furthermore, the roles of carbohydrates in assembly and allosteric effect, and conserved sulfur-containing residues in copper incorporation, were revealed. The precise structural information obtained in this study will accelerate our understanding of the molecular basis of hemocyanin and its future applications. Crystal Structure of the 3.8-MDa Respiratory Supermolecule Hemocyanin at 3.0 A Resolution.,Gai Z, Matsuno A, Kato K, Kato S, Khan MR, Shimizu T, Yoshioka T, Kato Y, Kishimura H, Kanno G, Miyabe Y, Terada T, Tanaka Y, Yao M Structure. 2015 Dec 1;23(12):2204-12. doi: 10.1016/j.str.2015.09.008. Epub 2015, Oct 22. PMID:26602184[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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