1jbg: Difference between revisions

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 ==Crystal Structure of MtaN, the Bacillus subtilis Multidrug Transporter Activator, N-terminus==
-<StructureSection load='1jbg' size='340' side='right' caption='[[1jbg]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
+<StructureSection load='1jbg' size='340' side='right'caption='[[1jbg]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1jbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JBG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1jbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JBG FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jbg RCSB], [http://www.ebi.ac.uk/pdbsum/1jbg PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbg OCA], [https://pdbe.org/1jbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jbg RCSB], [https://www.ebi.ac.uk/pdbsum/1jbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jbg ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MTA_BACSU MTA_BACSU]] Global transcriptional regulator that activates transcription of bmr and blt by binding directly to their promoter. Stimulates also the expression of the mta gene itself, ydfK and ymfE.<ref>PMID:10200972</ref> <ref>PMID:18502870</ref>
+[https://www.uniprot.org/uniprot/MTA_BACSU MTA_BACSU] Global transcriptional regulator that activates transcription of bmr and blt by binding directly to their promoter. Stimulates also the expression of the mta gene itself, ydfK and ymfE.<ref>PMID:10200972</ref> <ref>PMID:18502870</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jbg_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/1jbg_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jbg ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-MtaN (Multidrug Transporter Activation, N terminus) is a constitutive, transcriptionally active 109-residue truncation mutant, which contains only the N-terminal DNA-binding and dimerization domains of MerR family member Mta. The 2.75 A resolution crystal structure of apo-MtaN reveals a winged helix-turn-helix protein with a protruding 8-turn helix (alpha5) that is involved in dimerization by the formation of an antiparallel coiled-coil. The hydrophobic core and helices alpha1 through alpha4 are structurally homologous to MerR family member BmrR bound to DNA, whereas one wing (Wing 1) is shifted. Differences between the orientation of alpha5 with respect to the core and the revolution of the antiparallel coiled-coil lead to significantly altered conformations of MtaN and BmrR dimers. These shifts result in a conformation of MtaN that appears to be incompatible with the transcription activation mechanism of BmrR and suggest that additional DNA-induced structural changes are necessary.
-Crystal structure of MtaN, a global multidrug transporter gene activator.,Godsey MH, Baranova NN, Neyfakh AA, Brennan RG J Biol Chem. 2001 Dec 14;276(50):47178-84. Epub 2001 Oct 1. PMID:11581256<ref>PMID:11581256</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
 == References ==
 <references/>
@@ Line 30: / Line 24: @@
 </StructureSection>
 [[Category: Bacillus subtilis]]
-[[Category: Brennan, R G]]
+[[Category: Large Structures]]
-[[Category: Godsey, M H]]
+[[Category: Brennan RG]]
-[[Category: Neyfakh, A A]]
+[[Category: Godsey MH]]
-[[Category: Antiparallel coiled-coil]]
+[[Category: Neyfakh AA]]
-[[Category: Transcription]]
-[[Category: Winged helix-turn-helix]]