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==SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP== | |||
<StructureSection load='1hwq' size='340' side='right'caption='[[1hwq]]' scene=''> | |||
== Structural highlights == | |||
| | <table><tr><td colspan='2'>[[1hwq]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWQ FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwq OCA], [https://pdbe.org/1hwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwq RCSB], [https://www.ebi.ac.uk/pdbsum/1hwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwq ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
''' | == Publication Abstract from PubMed == | ||
== | |||
The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed. | The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed. | ||
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA.,Flinders J, Dieckmann T J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168<ref>PMID:11350168</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1hwq" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribozyme 3D structures|Ribozyme 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Dieckmann T]] | |||
[[Category: Flinders JC]] | |||
Latest revision as of 21:39, 29 November 2023
SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOPSOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP
Structural highlights
Publication Abstract from PubMedThe VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed. A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA.,Flinders J, Dieckmann T J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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