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[[Image:2e89.jpg|left|200px]]<br /><applet load="2e89" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2e89, resolution 2.50&Aring;" />
'''Crystal structure of Aquifex aeolicus TilS in a complex with ATP, Magnesium ion, and L-lysine'''<br />


==About this Structure==
==Crystal structure of Aquifex aeolicus TilS in a complex with ATP, Magnesium ion, and L-lysine==
2E89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=LYS:'>LYS</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E89 OCA].  
<StructureSection load='2e89' size='340' side='right'caption='[[2e89]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2e89]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E89 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e89 OCA], [https://pdbe.org/2e89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e89 RCSB], [https://www.ebi.ac.uk/pdbsum/2e89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e89 ProSAT], [https://www.topsan.org/Proteins/RSGI/2e89 TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TILS_AQUAE TILS_AQUAE] Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e8/2e89_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e89 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Kuratani, M.]]
[[Category: Kuratani M]]
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
[[Category: Takahashi S]]
[[Category: Takahashi, S.]]
[[Category: Yokoyama S]]
[[Category: Yokoyama, S.]]
[[Category: Yoshikawa Y]]
[[Category: Yoshikawa, Y.]]
[[Category: ATP]]
[[Category: LYS]]
[[Category: MG]]
[[Category: ligase]]
[[Category: national project on protein structural and functional analyses]]
[[Category: nppsfa]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rossmann-fold]]
[[Category: rsgi]]
[[Category: structural genomics]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:07:11 2008''

Latest revision as of 10:36, 9 October 2024

Crystal structure of Aquifex aeolicus TilS in a complex with ATP, Magnesium ion, and L-lysineCrystal structure of Aquifex aeolicus TilS in a complex with ATP, Magnesium ion, and L-lysine

Structural highlights

2e89 is a 4 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

TILS_AQUAE Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2e89, resolution 2.50Å

Drag the structure with the mouse to rotate

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