Gcn4: Difference between revisions

Latest revision as of 11:32, 12 March 2023

Function

Gcn4 or General control protein Gcn4 is a yeast transcriptional activator. It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. ^[1] Gcn4-pLI is a 33-residue peptide of the dimerization domain of Gcn4. See also Intrinsically Disordered Protein.

Structural highlights

Gcn4 and other family members have a DNA recognition motif consisting of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induces the transition of this region from unstructured to α-helical.

See Coiled coil.

3D Structures of Gcn4

Gcn4 3D Structures

Structure of Gcn4 leucine zipper domain complex with DNA (PDB entry 2dgc)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Hinnebusch AG. Gene-specific translational control of the yeast GCN4 gene by phosphorylation of eukaryotic initiation factor 2. Mol Microbiol. 1993 Oct;10(2):215-23. PMID:7934812

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Michal Harel, Alexander Berchansky, Karsten Theis

[1] Hinnebusch AG. Gene-specific translational control of the yeast GCN4 gene by phosphorylation of eukaryotic initiation factor 2. Mol Microbiol. 1993 Oct;10(2):215-23. PMID:7934812

[1]

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+<StructureSection load='2dgc' size='350' side='right' caption='Structure of Gcn4 leucine zipper domain complex with DNA  (PDB entry [[2dgc]])' scene=''54/540145/Biological_assembly/1''>
+== Function ==
-[[Image:2dgc.png|left|200px|thumb|Crystal structure of Gcn4 complex with DNA [[2dgc]]]]
+'''Gcn4''' or '''General control protein Gcn4''' is a yeast transcriptional activator.  It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. <ref>PMID:7934812</ref>  Gcn4-pLI is a 33-residue peptide of the dimerization domain of Gcn4.  See also [[Intrinsically Disordered Protein]].
-{{STRUCTURE_2dgc|  PDB=2dgc  | SIZE=400| SCENE= |right|CAPTION=Gcn4 complex with DNA [[2dgc]] }}
+== Structural highlights ==
+Gcn4 and other family members have a DNA recognition motif consisting of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding.  This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induces the transition of this region from unstructured to α-helical.
+See [[Coiled coil]].
-'''Gcn4''' is a yeast transcriptional activator.  It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a DNA recognition motif consists of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induce the transition of this region from unstructured to α-helical.  See also [[Intrinsically Disordered Protein]].
 == 3D Structures of Gcn4 ==
+[[Gcn4 3D Structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-[[2zta]], [[1w5i]], [[2b1f]], [[2b22]], [[2ag3]],  [[2oxj]], [[2oxk]], [[2r2v]] – yGcn4 - yeast <br />
-[[2ovn]], [[2l56]] – yGcn4 - NMR<br />
-[[1u2u]] – yGcn4 dimer - NMR<br />
-[[4dmd]], [[1kd8]], [[1kd9]], [[1kdd]] – yGcn4 dimer <br />
-[[4dme]] – yGcn4 trimer <br />
-[[3ck4]], [[3crp]], [[2ipz]] – yGcn4 tetramer <br />
-[[1gcl]], [[1swi]], [[1ce9]], [[1w5g]], [[1w5h]], [[1w5j]], [[1w5k]], [[1w5l]], [[1unt]], [[1unu]], [[1untv]], [[1unw]], [[1unx]], [[1uny]], [[1unz]], [[1uo0]], [[1uo1]], [[1uo2]], [[1uo3]], [[1uo4]], [[1uo5]], [[1u9f]], [[1u9g]], [[1u9h]], [[2bni]], [[2cce]], [[2ccf]], [[2ccn]], [[2ahp]], [[2hy6]], [[3i1g]], [[2wpy]], [[2wpz]], [[2wq0]], [[2wq1]], [[2wq2]], [[2wq3]], [[3k7z]], [[3gjp]], [[3m48]]  – yGcn4 (mutant)<br />
-[[1zii]], [[1zik]], [[1zil]] – yGcn4 dimer (mutant)<br />
-[[2o7h]], [[1gcm]], [[1zij]], [[1zim]], [[1piq]], [[1ij0]], [[1ij1]], [[1ij2]], [[1ij3]], [[1rb4]], [[1rb5]], [[1rb6]] – yGcn4 trimer (mutant)<br />
-[[2nrn]]  – yGcn4 tetramer (mutant)<br />
-[[1fmh]] – yGcn4 dimer (mutant) - NMR<br />
-[[1c94]] – yGcn4 retro<br />
-[[1ysa]], [[1dgc]], [[2dgc]] – yGcn4 dimer + DNA <br />
-===Gcn4 fusion proteins===
-[[1gk6]] – yGcn4/vimentin<br />
-[[2wg5]] – yGcn4/proteasome-activating nucleotidase<br />
-[[2wg6]] – yGcn4 (mutant)/proteasome-activating nucleotidase<br />
-[[2yny]], [[2ynz]], [[2yo0]], [[2yo1]], [[2yo2]], [[2yo3]], [[3zmf]] – yGcn4/inner membrane protein<br />
-[[3i5c]] – yGcn4/WSPR response regulator<br />
-[[3p8m]] – yGcn4/dynein<br />
-[[4g2k]] – yGcn4/virus envelope glycoprotein<br />
-[[1ld4]] – yGcn4 + virus coat protein + virus spike glycoprotein<br />
+== References ==
+<references/>
+[[Category:Topic Page]]