2igp: Difference between revisions

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-[[Image:2igp.jpg|left|200px]]<br /><applet load="2igp" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2igp, resolution 1.80&Aring;" />
-'''Crystal Structure of Hec1 CH domain'''<br />
-==Overview==
+==Crystal Structure of Hec1 CH domain==
-Kinetochores are multicomponent assemblies that connect chromosomal, centromeres to mitotic-spindle microtubules. The Ndc80 complex is an, essential core element of kinetochores, conserved from yeast to humans. It, is a rod-like assembly of four proteins- Ndc80p (HEC1 in humans), Nuf2p, Spc24p and Spc25p. We describe here the crystal structure of the most, conserved region of HEC1, which lies at one end of the rod and near the N, terminus of the polypeptide chain. It folds into a calponin-homology, domain, resembling the microtubule-binding domain of the, plus-end-associated protein EB1. We show that an Ndc80p-Nuf2p heterodimer, binds microtubules in vitro. The less conserved, N-terminal segment of, Ndc80p contributes to the interaction and may be a crucial regulatory, element. We propose that the Ndc80 complex forms a direct link between, kinetochore core components and spindle microtubules.
+<StructureSection load='2igp' size='340' side='right'caption='[[2igp]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2igp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IGP FirstGlance]. <br>
-IGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGP OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2igp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2igp OCA], [https://pdbe.org/2igp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2igp RCSB], [https://www.ebi.ac.uk/pdbsum/2igp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2igp ProSAT]</span></td></tr>
-The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment., Wei RR, Al-Bassam J, Harrison SC, Nat Struct Mol Biol. 2007 Jan;14(1):54-9. Epub 2006 Dec 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17195848 17195848]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NDC80_HUMAN NDC80_HUMAN] Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.<ref>PMID:9315664</ref> <ref>PMID:12351790</ref> <ref>PMID:14654001</ref> <ref>PMID:15235793</ref> <ref>PMID:15062103</ref> <ref>PMID:15239953</ref> <ref>PMID:14699129</ref> <ref>PMID:15548592</ref> <ref>PMID:16732327</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/2igp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2igp ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Harrison, S.C.]]
+[[Category: Harrison SC]]
-[[Category: Wei, R.R.]]
+[[Category: Wei RR]]
-[[Category: BME]]
-[[Category: alpha helices]]
-[[Category: calponin homology (ch) domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:36:12 2008''