3u1t: Difference between revisions

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[[Image:3u1t.png|left|200px]]


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==Haloalkane Dehalogenase, DmmA, of marine microbial origin==
The line below this paragraph, containing "STRUCTURE_3u1t", creates the "Structure Box" on the page.
<StructureSection load='3u1t' size='340' side='right'caption='[[3u1t]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3u1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U1T FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_3u1t|  PDB=3u1t  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u1t OCA], [https://pdbe.org/3u1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u1t RCSB], [https://www.ebi.ac.uk/pdbsum/3u1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u1t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6DND9_9CYAN Q6DND9_9CYAN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DmmA is a haloalkane dehalogenase (HLD) identified and characterized from the metagenomic DNA of a marine microbial consortium. Dehalogenase activity was detected with 1,3-dibromopropane as substrate, with steady-state kinetic parameters typical of HLDs (K(m) =0.24+/-0.05 mM, k(cat) =2.4+/-0.1 s-1). The 2.2-A crystal structure of DmmA revealed a fold and active site similar to other HLDs, but with a substantially larger active site binding pocket, suggestive of an ability to act on bulky substrates. This enhanced cavity was shown to accept a range of linear and cyclic substrates, suggesting that DmmA will contribute to the expanding industrial applications of HLDs.


===Haloalkane Dehalogenase, DmmA, of marine microbial origin===
Structure and activity of DmmA, a marine haloalkane dehalogenase.,Gehret JJ, Gu L, Geders TW, Brown WC, Gerwick L, Gerwick WH, Sherman DH, Smith JL Protein Sci. 2011 Nov 28. doi: 10.1002/pro.2009. PMID:22124946<ref>PMID:22124946</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3u1t" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_22124946}}, adds the Publication Abstract to the page
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 22124946 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22124946}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3u1t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U1T OCA].
 
==Reference==
<ref group="xtra">PMID:022124946</ref><references group="xtra"/>
[[Category: Haloalkane dehalogenase]]
[[Category: Unidentified]]
[[Category: Unidentified]]
[[Category: Gehret, J J]]
[[Category: Gehret JJ]]
[[Category: Smith, J L.]]
[[Category: Smith JL]]
[[Category: Alpha/beta-hydrolase]]
[[Category: Haloalkane dehalogenase]]
[[Category: Hydrolase]]

Latest revision as of 09:50, 27 November 2024

Haloalkane Dehalogenase, DmmA, of marine microbial originHaloalkane Dehalogenase, DmmA, of marine microbial origin

Structural highlights

3u1t is a 2 chain structure with sequence from Unidentified. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q6DND9_9CYAN

Publication Abstract from PubMed

DmmA is a haloalkane dehalogenase (HLD) identified and characterized from the metagenomic DNA of a marine microbial consortium. Dehalogenase activity was detected with 1,3-dibromopropane as substrate, with steady-state kinetic parameters typical of HLDs (K(m) =0.24+/-0.05 mM, k(cat) =2.4+/-0.1 s-1). The 2.2-A crystal structure of DmmA revealed a fold and active site similar to other HLDs, but with a substantially larger active site binding pocket, suggestive of an ability to act on bulky substrates. This enhanced cavity was shown to accept a range of linear and cyclic substrates, suggesting that DmmA will contribute to the expanding industrial applications of HLDs.

Structure and activity of DmmA, a marine haloalkane dehalogenase.,Gehret JJ, Gu L, Geders TW, Brown WC, Gerwick L, Gerwick WH, Sherman DH, Smith JL Protein Sci. 2011 Nov 28. doi: 10.1002/pro.2009. PMID:22124946[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Gehret JJ, Gu L, Geders TW, Brown WC, Gerwick L, Gerwick WH, Sherman DH, Smith JL. Structure and activity of DmmA, a marine haloalkane dehalogenase. Protein Sci. 2011 Nov 28. doi: 10.1002/pro.2009. PMID:22124946 doi:10.1002/pro.2009

3u1t, resolution 2.20Å

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