3sf5: Difference between revisions

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-[[Image:3sf5.png|left|200px]]
-<!--
+==Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H complex==
-The line below this paragraph, containing "STRUCTURE_3sf5", creates the "Structure Box" on the page.
+<StructureSection load='3sf5' size='340' side='right'caption='[[3sf5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3sf5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SF5 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.495&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_3sf5|  PDB=3sf5  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sf5 OCA], [https://pdbe.org/3sf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sf5 RCSB], [https://www.ebi.ac.uk/pdbsum/3sf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sf5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/UREF_HELPY UREF_HELPY] Required for maturation of urease via the functional incorporation of the urease nickel metallocenter (By similarity).[HAMAP-Rule:MF_01385]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Colonization of Helicobacter pylori in the acidic environment of the human stomach depends on the neutralizing activity of urease. Activation of apo-urease involves carboxylation of lysine 219 and insertion of two nickel ions. In H. pylori, this maturation process involves four urease accessory proteins as follows: UreE, UreF, UreG, and UreH. It is postulated that the apo-urease interacts with UreF, UreG, and UreH to form a pre-activation complex that undergoes GTP-dependent activation of urease. The crystal structure of the UreF-UreH complex reveals conformational changes in two distinct regions of UreF upon complex formation. First, the flexible C-terminal residues of UreF become ordered, forming an extra helix alpha10 and a loop structure stabilized by hydrogen bonds involving Arg-250. Second, the first turn of helix alpha2 uncoils to expose a conserved residue, Tyr-48. Substitution of R250A or Y48A in UreF abolishes the formation of the heterotrimeric complex of UreG-UreF-UreH and abolishes urease maturation. Our results suggest that the C-terminal residues and helix alpha2 of UreF are essential for the recruitment of UreG for the formation of the pre-activation complex. The molecular mass of the UreF-UreH complex determined by static light scattering was 116 +/- 2.3 kDa, which is consistent with the quaternary structure of a dimer of heterodimers observed in the crystal structure. Taking advantage of the unique 2-fold symmetry observed in both the crystal structures of H. pylori urease and the UreF-UreH complex, we proposed a topology model of the pre-activation complex for urease maturation.
-===Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H complex===
+Assembly of Preactivation Complex for Urease Maturation in Helicobacter pylori: CRYSTAL STRUCTURE OF UreF-UreH PROTEIN COMPLEX.,Fong YH, Wong HC, Chuck CP, Chen YW, Sun H, Wong KB J Biol Chem. 2011 Dec 16;286(50):43241-9. Epub 2011 Oct 19. PMID:22013070<ref>PMID:22013070</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3sf5" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_22013070}}, adds the Publication Abstract to the page
+*[[Urease accessory protein|Urease accessory protein]]
-(as it appears on PubMed at http://www.pubmed.gov), where 22013070 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_22013070}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3sf5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SF5 OCA].
-==Reference==
-<ref group="xtra">PMID:022013070</ref><references group="xtra"/>
 [[Category: Helicobacter pylori]]
-[[Category: Chen, Y W.]]
+[[Category: Large Structures]]
-[[Category: Fong, Y H.]]
+[[Category: Chen YW]]
-[[Category: Wong, K B.]]
+[[Category: Fong YH]]
-[[Category: Chaperone]]
+[[Category: Wong KB]]
-[[Category: Urease accessory protein]]
-[[Category: Uref]]
-[[Category: Ureh]]