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==Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (Form I, MV-H-SLAM(N102H/R108Y) fusion)== | |||
<StructureSection load='3alw' size='340' side='right'caption='[[3alw]], [[Resolution|resolution]] 3.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3alw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Measles_morbillivirus Measles morbillivirus], [https://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ALW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.55Å</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3alw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3alw OCA], [https://pdbe.org/3alw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3alw RCSB], [https://www.ebi.ac.uk/pdbsum/3alw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3alw ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HEMA_MEASE HEMA_MEASE] Attaches the virus to cell receptors and thereby initiating infection. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. May use human CD46 and/or SLAMF1 as receptors for viral entry into the cell. The high degree of interaction between H and MCP/CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis.<ref>PMID:9811778</ref> [https://www.uniprot.org/uniprot/Q9GJT3_SAGOE Q9GJT3_SAGOE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Measles virus, a major cause of childhood morbidity and mortality worldwide, predominantly infects immune cells using signaling lymphocyte activation molecule (SLAM) as a cellular receptor. Here we present crystal structures of measles virus hemagglutinin (MV-H), the receptor-binding glycoprotein, in complex with SLAM. The MV-H head domain binds to a beta-sheet of the membrane-distal ectodomain of SLAM using the side of its beta-propeller fold. This is distinct from attachment proteins of other paramyxoviruses that bind receptors using the top of their beta-propeller. The structure provides templates for antiviral drug design, an explanation for the effectiveness of the measles virus vaccine, and a model of the homophilic SLAM-SLAM interaction involved in immune modulations. Notably, the crystal structures obtained show two forms of the MV-H-SLAM tetrameric assembly (dimer of dimers), which may have implications for the mechanism of fusion triggering. | |||
Structure of the measles virus hemagglutinin bound to its cellular receptor SLAM.,Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y Nat Struct Mol Biol. 2011 Feb;18(2):135-41. Epub 2011 Jan 9. PMID:21217702<ref>PMID:21217702</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3alw" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]] | ||
[[Category: | == References == | ||
[[Category: Hashiguchi | <references/> | ||
[[Category: Kamishikiryo | __TOC__ | ||
[[Category: Kubota | </StructureSection> | ||
[[Category: Maenaka | [[Category: Large Structures]] | ||
[[Category: Maita | [[Category: Measles morbillivirus]] | ||
[[Category: Ose | [[Category: Saguinus oedipus]] | ||
[[Category: Yanagi | [[Category: Synthetic construct]] | ||
[[Category: Hashiguchi T]] | |||
[[Category: Kamishikiryo J]] | |||
[[Category: Kubota M]] | |||
[[Category: Maenaka K]] | |||
[[Category: Maita N]] | |||
[[Category: Ose T]] | |||
[[Category: Yanagi Y]] | |||
Latest revision as of 18:48, 4 October 2023
Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (Form I, MV-H-SLAM(N102H/R108Y) fusion)Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (Form I, MV-H-SLAM(N102H/R108Y) fusion)
Structural highlights
FunctionHEMA_MEASE Attaches the virus to cell receptors and thereby initiating infection. Binding of H protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. May use human CD46 and/or SLAMF1 as receptors for viral entry into the cell. The high degree of interaction between H and MCP/CD46 results in down-regulation of the latter from the surface of infected cells, rendering them more sensitive to c3b-mediated complement lysis.[1] Q9GJT3_SAGOE Publication Abstract from PubMedMeasles virus, a major cause of childhood morbidity and mortality worldwide, predominantly infects immune cells using signaling lymphocyte activation molecule (SLAM) as a cellular receptor. Here we present crystal structures of measles virus hemagglutinin (MV-H), the receptor-binding glycoprotein, in complex with SLAM. The MV-H head domain binds to a beta-sheet of the membrane-distal ectodomain of SLAM using the side of its beta-propeller fold. This is distinct from attachment proteins of other paramyxoviruses that bind receptors using the top of their beta-propeller. The structure provides templates for antiviral drug design, an explanation for the effectiveness of the measles virus vaccine, and a model of the homophilic SLAM-SLAM interaction involved in immune modulations. Notably, the crystal structures obtained show two forms of the MV-H-SLAM tetrameric assembly (dimer of dimers), which may have implications for the mechanism of fusion triggering. Structure of the measles virus hemagglutinin bound to its cellular receptor SLAM.,Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y Nat Struct Mol Biol. 2011 Feb;18(2):135-41. Epub 2011 Jan 9. PMID:21217702[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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