8fmb: Difference between revisions

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'''Unreleased structure'''


The entry 8fmb is ON HOLD
==Nodavirus RNA replication protein A polymerase domain, local refinement==
<StructureSection load='8fmb' size='340' side='right'caption='[[8fmb]], [[Resolution|resolution]] 6.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8fmb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Flock_House_virus Flock House virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FMB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fmb OCA], [https://pdbe.org/8fmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fmb RCSB], [https://www.ebi.ac.uk/pdbsum/8fmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fmb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RDRP_FHV RDRP_FHV] RNA-dependent RNA polymerase, which replicates the viral genome composed of 2 RNA segments, RNA1 and RNA2 (Probable). Does not need an exogenous primer (PubMed:24466277). Also possesses a terminal nucleotidyl transferase (TNTase) activity (PubMed:24466277). The TNTase catalyzes the addition of nucleotide to the 3'-end of plus- and minus-stranded RNAs, probably to repair the 3'-end nucleotide loss (PubMed:24466277). Forms the open necked connection to the cytosol of the virus-induced replication vesicles (PubMed:32690711, PubMed:36560715, PubMed:36693094). Mediates viral RNA1 recruitment (PubMed:17301137).<ref>PMID:17301137</ref> <ref>PMID:24466277</ref> <ref>PMID:32690711</ref> <ref>PMID:36560715</ref> <ref>PMID:36693094</ref> <ref>PMID:24466277</ref>


Authors:  
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
Description:  
== References ==
[[Category: Unreleased Structures]]
<references/>
__TOC__
</StructureSection>
[[Category: Flock House virus]]
[[Category: Large Structures]]
[[Category: Ahlquist P]]
[[Category: Broadberry R]]
[[Category: Grant T]]
[[Category: Horswill M]]
[[Category: Myers J]]
[[Category: Pennington J]]
[[Category: Rebolledo Viveros A]]
[[Category: Unchwaniwala N]]
[[Category: Zhan H]]
[[Category: Den Boon J]]

Latest revision as of 09:43, 19 June 2024

Nodavirus RNA replication protein A polymerase domain, local refinementNodavirus RNA replication protein A polymerase domain, local refinement

Structural highlights

8fmb is a 1 chain structure with sequence from Flock House virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 6.3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RDRP_FHV RNA-dependent RNA polymerase, which replicates the viral genome composed of 2 RNA segments, RNA1 and RNA2 (Probable). Does not need an exogenous primer (PubMed:24466277). Also possesses a terminal nucleotidyl transferase (TNTase) activity (PubMed:24466277). The TNTase catalyzes the addition of nucleotide to the 3'-end of plus- and minus-stranded RNAs, probably to repair the 3'-end nucleotide loss (PubMed:24466277). Forms the open necked connection to the cytosol of the virus-induced replication vesicles (PubMed:32690711, PubMed:36560715, PubMed:36693094). Mediates viral RNA1 recruitment (PubMed:17301137).[1] [2] [3] [4] [5] [6]

See Also

References

  1. ↑ Van Wynsberghe PM, Chen HR, Ahlquist P. Nodavirus RNA replication protein a induces membrane association of genomic RNA. J Virol. 2007 May;81(9):4633-44. PMID:17301137 doi:10.1128/JVI.02267-06
  2. ↑ Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y, Zhou X. Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS One. 2014 Jan 23;9(1):e86876. PMID:24466277 doi:10.1371/journal.pone.0086876
  3. ↑ Unchwaniwala N, Zhan H, Pennington J, Horswill M, den Boon JA, Ahlquist P. Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18680-18691. PMID:32690711 doi:10.1073/pnas.2006165117
  4. ↑ den Boon JA, Zhan H, Unchwaniwala N, Horswill M, Slavik K, Pennington J, Navine A, Ahlquist P. Multifunctional Protein A Is the Only Viral Protein Required for Nodavirus RNA Replication Crown Formation. Viruses. 2022 Dec 3;14(12):2711. PMID:36560715 doi:10.3390/v14122711
  5. ↑ Zhan H, Unchwaniwala N, Rebolledo-Viveros A, Pennington J, Horswill M, Broadberry R, Myers J, den Boon JA, Grant T, Ahlquist P. Nodavirus RNA replication crown architecture reveals proto-crown precursor and viral protein A conformational switching. Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2217412120. doi: , 10.1073/pnas.2217412120. Epub 2023 Jan 24. PMID:36693094 doi:http://dx.doi.org/10.1073/pnas.2217412120
  6. ↑ Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y, Zhou X. Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS One. 2014 Jan 23;9(1):e86876. PMID:24466277 doi:10.1371/journal.pone.0086876

8fmb, resolution 6.30Å

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