8fmb

Nodavirus RNA replication protein A polymerase domain, local refinementNodavirus RNA replication protein A polymerase domain, local refinement

Structural highlights

8fmb is a 1 chain structure with sequence from Flock House virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 6.3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RDRP_FHV RNA-dependent RNA polymerase, which replicates the viral genome composed of 2 RNA segments, RNA1 and RNA2 (Probable). Does not need an exogenous primer (PubMed:24466277). Also possesses a terminal nucleotidyl transferase (TNTase) activity (PubMed:24466277). The TNTase catalyzes the addition of nucleotide to the 3'-end of plus- and minus-stranded RNAs, probably to repair the 3'-end nucleotide loss (PubMed:24466277). Forms the open necked connection to the cytosol of the virus-induced replication vesicles (PubMed:32690711, PubMed:36560715, PubMed:36693094). Mediates viral RNA1 recruitment (PubMed:17301137).^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

References

↑ Van Wynsberghe PM, Chen HR, Ahlquist P. Nodavirus RNA replication protein a induces membrane association of genomic RNA. J Virol. 2007 May;81(9):4633-44. PMID:17301137 doi:10.1128/JVI.02267-06
↑ Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y, Zhou X. Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS One. 2014 Jan 23;9(1):e86876. PMID:24466277 doi:10.1371/journal.pone.0086876
↑ Unchwaniwala N, Zhan H, Pennington J, Horswill M, den Boon JA, Ahlquist P. Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18680-18691. PMID:32690711 doi:10.1073/pnas.2006165117
↑ den Boon JA, Zhan H, Unchwaniwala N, Horswill M, Slavik K, Pennington J, Navine A, Ahlquist P. Multifunctional Protein A Is the Only Viral Protein Required for Nodavirus RNA Replication Crown Formation. Viruses. 2022 Dec 3;14(12):2711. PMID:36560715 doi:10.3390/v14122711
↑ Zhan H, Unchwaniwala N, Rebolledo-Viveros A, Pennington J, Horswill M, Broadberry R, Myers J, den Boon JA, Grant T, Ahlquist P. Nodavirus RNA replication crown architecture reveals proto-crown precursor and viral protein A conformational switching. Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2217412120. doi: , 10.1073/pnas.2217412120. Epub 2023 Jan 24. PMID:36693094 doi:http://dx.doi.org/10.1073/pnas.2217412120
↑ Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y, Zhou X. Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS One. 2014 Jan 23;9(1):e86876. PMID:24466277 doi:10.1371/journal.pone.0086876

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Van Wynsberghe PM, Chen HR, Ahlquist P. Nodavirus RNA replication protein a induces membrane association of genomic RNA. J Virol. 2007 May;81(9):4633-44. PMID:17301137 doi:10.1128/JVI.02267-06

[2] Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y, Zhou X. Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS One. 2014 Jan 23;9(1):e86876. PMID:24466277 doi:10.1371/journal.pone.0086876

[3] Unchwaniwala N, Zhan H, Pennington J, Horswill M, den Boon JA, Ahlquist P. Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18680-18691. PMID:32690711 doi:10.1073/pnas.2006165117

[4] Boon JA, Zhan H, Unchwaniwala N, Horswill M, Slavik K, Pennington J, Navine A, Ahlquist P. Multifunctional Protein A Is the Only Viral Protein Required for Nodavirus RNA Replication Crown Formation. Viruses. 2022 Dec 3;14(12):2711. PMID:36560715 doi:10.3390/v14122711

[5] Zhan H, Unchwaniwala N, Rebolledo-Viveros A, Pennington J, Horswill M, Broadberry R, Myers J, den Boon JA, Grant T, Ahlquist P. Nodavirus RNA replication crown architecture reveals proto-crown precursor and viral protein A conformational switching. Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2217412120. doi: , 10.1073/pnas.2217412120. Epub 2023 Jan 24. PMID:36693094 doi:http://dx.doi.org/10.1073/pnas.2217412120

[6] Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y, Zhou X. Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS One. 2014 Jan 23;9(1):e86876. PMID:24466277 doi:10.1371/journal.pone.0086876

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