7ele: Difference between revisions

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-'''Unreleased structure'''
-The entry 7ele is ON HOLD  until Paper Publication
+==Cryo-EM structure of Arabidopsis DCL1 in complex with pre-miRNA 166f==
+<StructureSection load='7ele' size='340' side='right'caption='[[7ele]], [[Resolution|resolution]] 4.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7ele]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ELE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ELE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ele FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ele OCA], [https://pdbe.org/7ele PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ele RCSB], [https://www.ebi.ac.uk/pdbsum/7ele PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ele ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DCL1_ARATH DCL1_ARATH] Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Functions with DRB1/HYL1 and SERRATE proteins for accurate pri-miRNAs to miRNAs processing. Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved in the processing of natural siRNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving 24 nucleotide nat-siRNAs into 21 nucleotide nat-siRNAs. Can produce RDR6-dependent endogenous ta-siRNAs derived from TAS1 and TAS2. Required for the production of 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). Acts redundantly with DICER-LIKE 3 (DCL3) to promote flowering via repression of FLOWERING LOCUS C (FLC). Represses antiviral RNA silencing through negative regulation of the expression of DCL4 and DCL3.<ref>PMID:15821876</ref> <ref>PMID:16040244</ref> <ref>PMID:16428603</ref> <ref>PMID:17579240</ref> <ref>PMID:18003861</ref> <ref>PMID:18632569</ref> <ref>PMID:18799732</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+MicroRNAs (miRNAs) are short non-coding RNAs that inhibit the expression of target genes by directly binding to their mRNAs. In animals, pri-miRNAs are cleaved by Drosha to generate pre-miRNAs, which are subsequently cleaved by Dicer to generate mature miRNAs. Instead of being cleaved by two different enzymes, both cleavages in plants are performed by Dicer-like 1 (DCL1). With a similar domain architecture as human Dicer, it is mysterious how DCL1 recognizes pri-miRNAs and performs two cleavages sequentially. Here, we report the single-particle cryo-electron microscopy structures of Arabidopsis DCL1 complexed with a pri-miRNA and a pre-miRNA, respectively, in cleavage-competent states. These structures uncover the plasticity of the PAZ domain, which is critical for the recognition of both pri-miRNA and pre-miRNA. These structures suggest that the helicase module serves as an engine that transfers the substrate between two sequential cleavage events. This study lays a foundation for dissecting the regulation mechanism of miRNA biogenesis in plants and provides insights into the dicing state of human Dicer.
-Authors:
+Structural basis of microRNA processing by Dicer-like 1.,Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y Nat Plants. 2021 Oct;7(10):1389-1396. doi: 10.1038/s41477-021-01000-1. Epub 2021 , Sep 30. PMID:34593993<ref>PMID:34593993</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7ele" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Feng Y]]
+[[Category: Ke H]]
+[[Category: Wei X]]