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==Cholera toxin classical B-pentamer in complex with Lewis-y== | ==Cholera toxin classical B-pentamer in complex with Lewis-y== | ||
<StructureSection load='5elb' size='340' side='right' caption='[[5elb]], [[Resolution|resolution]] 1.08Å' scene=''> | <StructureSection load='5elb' size='340' side='right'caption='[[5elb]], [[Resolution|resolution]] 1.08Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5elb]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ELB OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5elb]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1 Vibrio cholerae O1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ELB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ELB FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.08Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5elb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5elb OCA], [https://pdbe.org/5elb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5elb RCSB], [https://www.ebi.ac.uk/pdbsum/5elb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5elb ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q57193_VIBCL Q57193_VIBCL] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 16: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5elb" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5elb" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Cholera toxin 3D structures|Cholera toxin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Vibrio cholerae O1]] | ||
[[Category: | [[Category: Burschowsky D]] | ||
[[Category: | [[Category: Heggelund JE]] | ||
[[Category: | [[Category: Krengel U]] | ||
Latest revision as of 07:02, 21 November 2024
Cholera toxin classical B-pentamer in complex with Lewis-yCholera toxin classical B-pentamer in complex with Lewis-y
Structural highlights
FunctionPublication Abstract from PubMedCholera is the prime example of blood-group-dependent diseases, with individuals of blood group O experiencing the most severe symptoms. The cholera toxin is the main suspect to cause this relationship. We report the high-resolution crystal structures (1.1-1.6 A) of the native cholera toxin B-pentamer for both classical and El Tor biotypes, in complexes with relevant blood group determinants and a fragment of its primary receptor, the GM1 ganglioside. The blood group A determinant binds in the opposite orientation compared to previously published structures of the cholera toxin, whereas the blood group H determinant, characteristic of blood group O, binds in both orientations. H-determinants bind with higher affinity than A-determinants, as shown by surface plasmon resonance. Together, these findings suggest why blood group O is a risk factor for severe cholera. High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.,Heggelund JE, Burschowsky D, Bjornestad VA, Hodnik V, Anderluh G, Krengel U PLoS Pathog. 2016 Apr 15;12(4):e1005567. doi: 10.1371/journal.ppat.1005567., eCollection 2016 Apr. PMID:27082955[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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