NADPH dehydrogenase: Difference between revisions

Latest revision as of 13:29, 15 July 2024

Function

NADPH dehydrogenase or old yellow enzyme or NADH:flavin oxidoreductase/NADH oxidase or prostaglandin F2A synthase or chromate reductase (OYE) catalyzes the conversion of NADPH, H+ and acceptor to NADP+ and reduced acceptor^[1]. See NAD(P)H. The acceptor is a quinone. OYE is a flavoenzyme with two cofactors: FAD and FMN.

NADPH dehydrogenase quinone 1 (NQO1) reduces quinones to hydroquinones.
NADPH dehydrogenase quinone 2 (NQO2) catayzes electron transfer from NADH to ubiquinone in the mitochondrial respiratory chain^[2].

Structural highlights

^[3]. Water molecules are shown as red spheres.

Structure of yeast NADPH dehydrogenase complex with FMN, p-hydroxybenzaldehyde and Mg+2 ion (green) (PDB code 1k03).

Drag the structure with the mouse to rotate

3D Structures of NADPH dehydrogenase3D Structures of NADPH dehydrogenase

Updated on 15-July-2024

ReferencesReferences

↑ Ryu JY, Suh KH, Chung YH, Park YM, Chow WS, Park YI. NADPH dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light. Mol Cells. 2003 Apr 30;15(2):240-4. PMID:12803488
↑ Feng Y, Li W, Li J, Wang J, Ge J, Xu D, Liu Y, Wu K, Zeng Q, Wu JW, Tian C, Zhou B, Yang M. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature. 2012 Nov 15;491(7424):478-82. doi: 10.1038/nature11541. Epub 2012 Oct 21. PMID:23086143 doi:http://dx.doi.org/10.1038/nature11541
↑ Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V. The role of glutamine 114 in old yellow enzyme. J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:11668181 doi:10.1074/jbc.M108453200

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Ryu JY, Suh KH, Chung YH, Park YM, Chow WS, Park YI. NADPH dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light. Mol Cells. 2003 Apr 30;15(2):240-4. PMID:12803488

[2] Feng Y, Li W, Li J, Wang J, Ge J, Xu D, Liu Y, Wu K, Zeng Q, Wu JW, Tian C, Zhou B, Yang M. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature. 2012 Nov 15;491(7424):478-82. doi: 10.1038/nature11541. Epub 2012 Oct 21. PMID:23086143 doi:http://dx.doi.org/10.1038/nature11541

[3] Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V. The role of glutamine 114 in old yellow enzyme. J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:11668181 doi:10.1074/jbc.M108453200

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@@ Line 3: / Line 3: @@
 == Function ==
-'''NADPH dehydrogenase''' or '''old yellow enzyme''' (OYE) catalyzes the conversion of NADPH, H+ and acceptor to NADP+ and reduced acceptor<ref>PMID:12803488</ref>.  See  [[NAD(P)H]].  The acceptor is a quinone.  OYE is a flavoenzyme with two cofactors: FAD and FMN.
+'''NADPH dehydrogenase''' or '''old yellow enzyme''' or '''NADH:flavin oxidoreductase/NADH oxidase''' or '''prostaglandin F2A synthase''' or '''chromate reductase''' (OYE) catalyzes the conversion of NADPH, H+ and acceptor to NADP+ and reduced acceptor<ref>PMID:12803488</ref>.  See  [[NAD(P)H]].  The acceptor is a quinone.  OYE is a flavoenzyme with two cofactors: FAD and FMN.
+*'''NADPH dehydrogenase quinone 1''' (NQO1) reduces quinones to hydroquinones.
+*'''NADPH dehydrogenase quinone 2''' (NQO2) catayzes electron transfer from NADH to ubiquinone in the mitochondrial respiratory chain<ref>PMID:23086143</ref>.
 == Structural highlights ==
 <scene name='59/595221/Cv/5'>OYE cofactor FMN interacts with the ligand in active site</scene><ref>PMID:11668181</ref>. Water molecules are shown as red spheres.
@@ Line 16: / Line 18: @@
 *NADPH dehydrogenase
+**[[7blf]] – OYE + FMN - fungus<br />
 **[[1oyc]], [[1oya]] – yOYE + FMN - yeast<br />
 **[[1oyb]] – yOYE + FMN + p-hydroxybenzaldehyde <br />
 **[[3tjl]] – SsOYE + FMN + malonate - ''Scheffersomyces stipitis'' <br />
+**[[6s32]] – OYE + FMN + benzamidine – ''Chroococcidiopsis thermalis''<br />
+**[[6s31]] – GsOYE + FMN + benzaldehyde – ''Galdieria sulphuraria''<br />
+**[[6s0g]], [[6s23]] – GsOYE + FMN <br />
+**[[6agz]] – OYE + FMN - ''Pichia''<br />
+**[[3kru]], [[3krz]] – OYE + FMN – ''Thermoanaerobacter pseudethanolicus''<br />
+**[[3atz]] – TcOYE + FMN + benzaldehyde – ''Trypanosoma cruzi''<br />
+**[[3aty]] – TcOYE + FMN <br />
+**[[2gou]], [[2gqa]] – SoOYE + FMN – ''Shewanella oneidensis''<br />
+**[[2gq9]] – SoOYE + FMN + benzaldehyde <br />
+**[[7o0t]] – OYE + FMN – ''Chloroflexus aggregans''<br />
+**[[3hgj]] – TsOYE + FMN + benzaldehyde – ''Thermus scotoductus''<br />
+**[[3hf3]] – TsOYE + FMN <br />
 *NADPH dehydrogenase 1
@@ Line 45: / Line 60: @@
 **[[5v4p]] – yOYE 3 + FMN + p-hydroxybenzaldehyde <br />
 **[[5v4v]] – yOYE 3 + FMN + nicotinamide <br />
+*NADPH dehydrogenase 4
+**[[4b5n]] – SoOYE 4 + FMN <br />
+*NADPH dehydrogenase quinone 1
+**[[5a4k]] – hNQO1 (mutant) + FAD - human <br />
+**[[5fuq]], [[4cet]] – hNQO1 (mutant) + FAD + dicoumarol <br />
+**[[4cf6]] – hNQO1 (mutant) + FAD + cibacron blue <br />
 }}