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==RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution==
==RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution==
<StructureSection load='4tnh' size='340' side='right' caption='[[4tnh]], [[Resolution|resolution]] 4.90&Aring;' scene=''>
<StructureSection load='4tnh' size='340' side='right'caption='[[4tnh]], [[Resolution|resolution]] 4.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4tnh]] is a 40 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNH FirstGlance]. <br>
<table><tr><td colspan='2'>[[4tnh]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=OEX:CA-MN4-O5+CLUSTER'>OEX</scene>, <scene name='pdbligand=PHO:PHEOPHYTIN+A'>PHO</scene>, <scene name='pdbligand=PL9:2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE'>PL9</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.900007&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PHO:PHEOPHYTIN+A'>PHO</scene>, <scene name='pdbligand=PL9:2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE'>PL9</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tni|4tni]], [[4tnj|4tnj]], [[4tnk|4tnk]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnh OCA], [https://pdbe.org/4tnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnh RCSB], [https://www.ebi.ac.uk/pdbsum/4tnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnh ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photosystem_II Photosystem II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.9 1.10.3.9] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnh OCA], [http://pdbe.org/4tnh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnh RCSB], [http://www.ebi.ac.uk/pdbsum/4tnh PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PSBL_THEEB PSBL_THEEB]] Required for PSII activity (By similarity). [[http://www.uniprot.org/uniprot/YCF12_THEEB YCF12_THEEB]] A core subunit of photosystem II (PSII).[HAMAP-Rule:MF_01329] [[http://www.uniprot.org/uniprot/PSBA1_THEEB PSBA1_THEEB]] This is one of the two reaction center proteins of photosystem II. [[http://www.uniprot.org/uniprot/PSBX_THEEB PSBX_THEEB]] Involved in the binding and/or turnover of quinones at the Q(B) site of Photosystem II.<ref>PMID:11230572</ref>  [[http://www.uniprot.org/uniprot/CY550_THEEB CY550_THEEB]] Low-potential cytochrome c that plays a role in the oxygen-evolving complex of photosystem II. It is not essential for growth under normal conditions but is required under low CO(2) concentrations.[HAMAP-Rule:MF_01378] [[http://www.uniprot.org/uniprot/PSBO_THEEB PSBO_THEEB]] MSP binds to a putative Mn-binding protein and keeps 2 of the 4 Mn-atoms associated with PSII (By similarity). [[http://www.uniprot.org/uniprot/PSBF_THEEB PSBF_THEEB]] This b-type cytochrome is tightly associated with the reaction center of photosystem II and possibly is part of the water-oxidation complex (By similarity).[HAMAP-Rule:MF_00643] [[http://www.uniprot.org/uniprot/PSBJ_THEEB PSBJ_THEEB]] This protein is a component of the reaction center of photosystem II (By similarity). [[http://www.uniprot.org/uniprot/PSBZ_THEEB PSBZ_THEEB]] Controls the interaction of photosystem II (PSII) cores with the light-harvesting antenna. May also aid in binding of PsbK, Ycf12 and the oxygen-evolving complex to PSII, at least in vitro.<ref>PMID:17967798</ref> [[http://www.uniprot.org/uniprot/PSBT_THEEB PSBT_THEEB]] Seems to play a role in the dimerization of PSII.<ref>PMID:15653799</ref> [[http://www.uniprot.org/uniprot/PSBU_THEEB PSBU_THEEB]] Stabilizes the structure of photosystem II oxygen-evolving complex (OEC), the ion environment of oxygen evolution and protects the OEC against heat-induced inactivation (By similarity).[HAMAP-Rule:MF_00589] [[http://www.uniprot.org/uniprot/PSBI_THEEB PSBI_THEEB]] This protein is a component of the reaction center of photosystem II.[HAMAP-Rule:MF_01316] [[http://www.uniprot.org/uniprot/PSBK_THEEB PSBK_THEEB]] This protein is a component of the reaction center of photosystem II.[HAMAP-Rule:MF_00441] [[http://www.uniprot.org/uniprot/PSBE_THEEB PSBE_THEEB]] This b-type cytochrome is tightly associated with the reaction center of photosystem II and possibly is part of the water-oxidation complex.[HAMAP-Rule:MF_00642]
[https://www.uniprot.org/uniprot/PSBE_THEVB PSBE_THEVB] This b-type cytochrome is tightly associated with the reaction center of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.[HAMAP-Rule:MF_00642]<ref>PMID:20558739</ref> <ref>PMID:21367867</ref> <ref>PMID:25006873</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
*[[Photosystem II|Photosystem II]]
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
*[[Photosystem II 3D structures|Photosystem II 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Photosystem II]]
[[Category: Large Structures]]
[[Category: Thermosynechococcus elongatus]]
[[Category: Thermosynechococcus vestitus BP-1]]
[[Category: Adams, P D]]
[[Category: Adams PD]]
[[Category: Alonso-Mori, R]]
[[Category: Alonso-Mori R]]
[[Category: Bergmann, U]]
[[Category: Bergmann U]]
[[Category: Bogan, M J]]
[[Category: Bogan MJ]]
[[Category: Boutet, S]]
[[Category: Boutet S]]
[[Category: Brewster, A]]
[[Category: Brewster A]]
[[Category: Chatterjee, R]]
[[Category: Chatterjee R]]
[[Category: DiFiore, D]]
[[Category: DiFiore D]]
[[Category: Echols, N]]
[[Category: Echols N]]
[[Category: Fry, A R]]
[[Category: Fry AR]]
[[Category: Gallo, E]]
[[Category: Gallo E]]
[[Category: Gildea, R J]]
[[Category: Gildea RJ]]
[[Category: Glatzel, P]]
[[Category: Glatzel P]]
[[Category: Gloeckner, C]]
[[Category: Gloeckner C]]
[[Category: Gul, S]]
[[Category: Gul S]]
[[Category: Han, G]]
[[Category: Han G]]
[[Category: Hattne, J]]
[[Category: Hattne J]]
[[Category: Hellmich, J]]
[[Category: Hellmich J]]
[[Category: Ibrahim, M]]
[[Category: Ibrahim M]]
[[Category: Kern, J]]
[[Category: Kern J]]
[[Category: Koglin, J E]]
[[Category: Koglin JE]]
[[Category: Koroidov, S]]
[[Category: Koroidov S]]
[[Category: Laksmono, H]]
[[Category: Laksmono H]]
[[Category: Lampe, A]]
[[Category: Lampe A]]
[[Category: Lassalle-Kaiser, B]]
[[Category: Lassalle-Kaiser B]]
[[Category: Messerschmidt, M]]
[[Category: Messerschmidt M]]
[[Category: Messinger, J]]
[[Category: Messinger J]]
[[Category: Milathianaki, D]]
[[Category: Milathianaki D]]
[[Category: Sauter, N K]]
[[Category: Sauter NK]]
[[Category: Seibert, M M]]
[[Category: Seibert MM]]
[[Category: Sierra, R G]]
[[Category: Sierra RG]]
[[Category: Skinner, D E]]
[[Category: Skinner DE]]
[[Category: Sokaras, D]]
[[Category: Sokaras D]]
[[Category: Stan, C A]]
[[Category: Stan CA]]
[[Category: Tran, R]]
[[Category: Tran R]]
[[Category: Uhlig, J]]
[[Category: Uhlig J]]
[[Category: Weng, T C]]
[[Category: Weng T-C]]
[[Category: Williams, G J]]
[[Category: Williams GJ]]
[[Category: Yachandra, V K]]
[[Category: Yachandra VK]]
[[Category: Yano, J]]
[[Category: Yano J]]
[[Category: Zouni, A]]
[[Category: Zouni A]]
[[Category: Zwart, P H]]
[[Category: Zwart PH]]
[[Category: Electron transport]]
[[Category: Membrane protein]]
[[Category: Photosynthesis]]
[[Category: Water oxidation]]
[[Category: X-ray free electron laser]]

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